Biodetection data: Using odor signature in white-tailed deer associated with infection by Chronic Wasting Disease prions

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Entity_and_Attribute_Information:

Overview_Description:

Entity_and_Attribute_Overview:

Below you will find a list and description of the files included in this data publication.

VARIABLE DESCRIPTION FILE (1)

1. \Data\_variable_descriptions.csv: Comma-separated values (CSV) file containing a list and description of variables found in all data files. (This same content is also provided as a separate worksheet within each of the XLSX files included in this data package and are also fully defined in those file descriptions below.)

Columns include:
Filename = name of data file
Worksheet = name of worksheet within the specified file
Variable = name of variable
Description = description of variable



DATA FILES (3)

1. \Data\Demo_learned_responses_Jan-Aug2021.xlsx: Microsoft Excel Open XML spreadsheet (XLSX) file containing multiple worksheets that contain the following data: i) accuracy of dogs during early training using a passive sit alert to indicate the location of the CWD-positive sample in comparison to ‘odorless’ propylene glycol (PG); ii) accuracy of dogs in training using a single panel with four different CWD-positive samples; iii) accuracy of dogs in training using a 80 Cohort 1 CWD-negative fecal samples (4 in each of 20 trials) and 20 Cohort 1 (1 in each of 20 trials) CWD-positive samples; and iv) accuracy of dogs in training using a 80 CWD-negative fecal samples (4 in each of 20 trials) and 20 (1 in each of 20 trials) CWD-positive samples from Cohorts 1 and 2. Study done from January to August 2021.

This file contains 5 worksheets, which are described below.

Worksheet 1 - 'Variable Descriptions': list and description of the variables provided in this data file.
Variables include:
Worksheet = name of worksheet
Variable = name of variable
Description = description of variable


Worksheet 2 - '4 CS+ vs PG': accuracy of dogs during early training using a passive sit alert to indicate the location of the CWD-positive sample in comparison to ‘odorless’ propylene glycol (PG).

Variables include:

A.I.D. = animal identification number

Date = date of training or testing (mm/dd/yyyy)

Blind = double-blind status (trial run with handler and dog blind to location of CS+) (Y=yes, N=no)

Paired = was fecal sample paired with food or tennis ball skin (Y=yes, N=no)

Trials = trial number (1-20)

Reward = rewarded trial or non-rewarded trial (extinction or generalization) (R=reward, E=extinction, G=generalization)

Position = location of the fecal sample collected from white-tailed deer (WTD) confirmed to be infected with CWD (confirmed positive) (1-5)

CS-_1 = location of the first propylene glycol (PG) sample

CS-_2 = location of the second propylene glycol (PG) sample

CS-_3 = location of the third propylene glycol (PG) sample

CS-_4 = location of the fourth propylene glycol (PG) sample

CS+ = identification of the CWD infected WTD that provided sample

Hit = correct choice as shown by "sit" alert at the box containing the CS+

Miss = incorrect choice as shown by "sit" alert at the box containing any CS-

Choice = fecal sample in the box chosen whether correctly or incorrectly

Alert = position of the box where the dog performed the "sit" alert whether it was a correct or incorrect response (1-5)

Result = result of the trial (hit or miss)

Sample Origin = origin (e.g., region or geographic location) of the fecal sample in the box chosen correctly

Sex = was the donor male (M) or female (F) (or UNK=unknown)

CWD Status = CWD infection status; shown by tissue tested and result for correct choices ('POS LN'=confirmed positive using lymph nodes, Neg=nonconfirmed for CWD samples, 'Pos'=confirmed for CWD samples, and 'Pos Brain & LN'=confirmed positive using brain and lymph nodes)


Worksheet 3 - '4 CS+ vs 4 CS-': accuracy of dogs in training using a single panel with four different CWD-positive samples.

Variables descriptions for Worksheets 3-5 are the same. Variables include:

A.I.D. = animal identification number

Date = date of training or testing (mm/dd/yyyy)

Blind = double-blind status (trial run with handler and dog blind to location of CS+) (Y=yes, N=no)

Paired = was fecal sample paired with food or tennis ball skin (Y=yes, N=no)

Trials = trial number (1-20)

Reward = rewarded trial or non-rewarded trial (extinction or generalization) (R=reward, G=generalization, E=extinction)

Position = location of the fecal sample collected from white-tailed deer (WTD) confirmed to be infected with CWD (confirmed positive) (1-5)

CS-_1 = location of the first fecal sample from unconfirmed WTD

CS-_2 = location of the second fecal sample from unconfirmed WTD

CS-_3 = location of the third fecal sample from unconfirmed WTD

CS-_4 = location of the fourth fecal sample from unconfirmed WTD

CS+ = identification of the CWD infected WTD that provided sample

Hit = correct choice as shown by "sit" alert at the box containing the CS+

Miss = incorrect choice as shown by "sit" alert at the box containing any CS-

Choice = fecal sample in the box chosen whether correctly or incorrectly

Alert = position of the box where the dog performed the "sit" alert whether it was a correct or incorrect response (1-5)

Result = result of the trial (hit or miss)

Sample Origin = origin (e.g., region or geographic location) of the fecal sample in the box chosen correctly

Sex = was the donor male (M) or female (F)

CWD Status = CWD infection status; shown by tissue tested and result for correct choices ('POS LN'=confirmed positive using lymph nodes, Neg=nonconfirmed for CWD samples, 'Pos'=confirmed for CWD samples, and 'Pos Brain & LN'=confirmed positive using brain and lymph nodes)


Worksheet 4 - '20 CS+ vs 80 CS-': accuracy of dogs in training using a 80 Cohort 1 CWD-negative fecal samples (4 in each of 20 trials) and 20 Cohort 1 (1 in each of 20 trials) CWD-positive samples. (See variable descriptions provided for Worksheet 3 above.)


Worksheet 5 - '20 CS+ vs 80 CS- TN': accuracy of dogs in training using a 80 CWD-negative fecal samples (4 in each of 20 trials) and 20 (1 in each of 20 trials) CWD-positive samples from Cohorts 1 and 2. (See variable descriptions provided for Worksheet 3 above.)



2. \Data\Fecal_Exp_Analysis_Aug-Sep2021.xlsx: XLSX file containing data on the accuracy of dogs being tested using 12 novel CWD-negative fecal samples (4 in each of 3 of 20 trials) and 3 novel (1 in each of 3 of 20 trials) CWD-positive samples from Cohort 1 during generalization trials. Study was done from August 31, 2021 through September 01, 2021.

Variables include:

A.I.D. = animal identification number

Date = date of training or testing (mm/dd/yyyy)

Blind = double-blind status (trial run with handler and dog blind to location of CS+) (Y=yes)

Paired = was fecal sample paired with food or tennis ball skin (Y=yes, N=no)

Trials = trial number (1-20)

Reward = rewarded trial or non-rewarded trial (extinction or generalization) (R=reward, G=generalization, E=extinction)

Position = location of the fecal sample collected from white-tailed deer confirmed by PCR to contain avian influenza virus (confirmed positive) (1-5)

CS-_1 = first fecal sample from noninfected white-tailed deer location

CS-_2 = second fecal sample from noninfected white-tailed deer location

CS-_3 = third fecal sample from noninfected white-tailed deer location

CS-_4 = fourth fecal sample from noninfected white-tailed deer location

CS+ = Identification of the infected white-tailed deer that provided sample

Hit = correct choice by scratch alert on the box containing the CS+

Miss = incorrect choice by scratch alert on the box containing any CS-

Choice = fecal sample in the box chosen incorrectly

Alert = position of box dog sat at whether correctly or incorrectly (1-5)

Result = result of the trial (hit or miss)

Sample Origin = Region where white-tailed deer herd depopulation occurred

Sex = was the donor male (M) or female (F) (UNK=unknown)

Age (months) = age of the donor in months (UNK=unknown)

CWD Status = CWD infection status; shown by tissue tested and result for correct choices ('POS LN'=confirmed positive using lymph nodes, Neg=nonconfirmed for CWD samples, 'Pos'=confirmed for CWD samples, and 'Pos Brain & LN'=confirmed positive using brain and lymph nodes)



3. \Data\GI_Tract_Exp_Analysis_Oct2022.xlsx: XLSX file containing data on the accuracy of dogs being tested in their ability to generalize to a tissue (i.e., small intestine) from novel donors using 8 CWD-negative small intestine samples (8 in each of 2 generalization trials of 20 trials) and 2 (2 in each of 2 generalization trials of 20 trials) CWD-positive small intestine samples from Cohort 3. Study was done from October 11-13, 2022 and October 25-27, 2022.

Variables include:

A.I.D. = animal identification number

Date = date of training or testing (mm/dd/yyyy)

Blind = double-blind status (trial run with handler and dogt blind to location of CS+) (Y=yes)

Trials = trial number (1-20)

Reward = rewarded trial or non-rewarded trial (extinction or generalization) (R=reward, G=generalization, E=extinction)

Position = location of the fecal sample collected from white-tailed deer confirmed by PCR to contain avian influenza virus (confirmed positive) (1-5)

CS-_1 = first fecal sample from noninfected white-tailed deer location

CS-_2 = second fecal sample from noninfected white-tailed deer location

CS-_3 = third fecal sample from noninfected white-tailed deer location

CS-_4 = fourth fecal sample from noninfected white-tailed deer location

CS+ = Identification of the infected white-tailed deer that provided sample

Hit = correct choice by scratch alert on the box containing the CS+

Miss = incorrect choice by scratch alert on the box containing any CS-

Choice = fecal sample in the box chosen incorrectly

Alert = position of box dog sat at whether correctly or incorrectly (1-5)

Result = result of the trial (hit or miss)

Tissue Type = describes if sample is collected from the colon (Colon) or the small intestine (Intestine)

Sample Origin = Region where white-tailed deer herd depopulation occurred (Ames=Ames Plantation, 'Pres Island'=President Island, 'SW TN'=southwestern Tennessee)

Sex = was the donor male (M) or female (F)

Age (months) = the age of the donor in months (if known)

CWD Status = CWD infection status; shown by tissue tested and result for correct choices ('POS LN'=confirmed positive using lymph nodes, Neg=nonconfirmed for CWD samples, 'Pos'=confirmed for CWD samples, and 'Pos Brain & LN'=confirmed positive using brain and lymph nodes)



SUPPLEMENTAL FILES (1)

1. \Supplements\Golden_etal_2024_Tables1-2.pdf: Portable Document Format file containing Tables 1 and 2 from Golden et al. (2024). Table 1 contains an overview of the four types of training used in this study and Table 2 contains specific details about the demonstration and experiments in this study.

Entity_and_Attribute_Detail_Citation:

Golden, Glen J.; Ramirez, Elizabeth A.; Stevens, Hayley N.; Bourbois, Jennifer; Grove, Daniel M.; Bowen, Richard A.; DeLiberto, Thomas J.; Kimball, Bruce A. 2024. Biodetection of an odor signature in white-tailed deer associated with infection by chronic wasting disease prions. PLOS ONE. https://doi.org/10.1101/2024.04.24.590903

Metadata_Reference_Information:

Metadata_Date: 20240709

Metadata_Contact:

Contact_Information:

Contact_Organization_Primary:

Contact_Organization: Colorado State University; USDA, APHIS, Wildlife Service, National Wildlife Research Center

Contact_Person: Glen J. Golden

Contact_Position: Research Scientist

Contact_Address:

Address_Type: mailing and physical

Address: Colorado State University

Address: 1682 Campus Delivery

City: Fort Collins

State_or_Province: CO

Postal_Code: 80523-1683

Country: USA

Contact_Voice_Telephone: 267-884-6941

Contact_Electronic_Mail_Address: glen.golden@colostate.edu

Contact Instructions: This contact information was current as of original publication date. For current information see Contact Us page on: https://doi.org/10.2737/RDS.

Metadata_Standard_Name: FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata

Metadata_Standard_Version: FGDC-STD-001.1-1999