Extraction and estimation of the quantity of calcium oxalate crystals in the foliage of conifer and hardwood trees

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Entity_and_Attribute_Information:

Overview_Description:

Entity_and_Attribute_Overview:

Below you will find a list and description of the files included in this data publication.

Unit abbreviations used:

µmol g⁻¹ calc FW = micromoles per gram calculated fresh weight
µmol g⁻¹ FW = micromoles per gram fresh weight


VARIABLE DESCRIPTION FILE (1)

1. \Data\_variable_descriptions.csv: Comma-separated values (CSV) file containing a list and description of variables found in all data files. (A description of these variables is also provided in the metadata below.)

Columns include:

Filename = name of data file
Variable = name of variable
Units = units (if applicable)
Precision = precision (if applicable)
Description = description of variable
Important Notes = Additional notes to interpret that variable
References = Relevant references for some variables


DATA FILES (2)
For both files, -888.88 denotes data not available (na) and -999.99 denotes no analysis for that sample.

1. \Data\Minocha_CaOX_Method_data_Exp1.csv: CSV file containing comparative estimations of soluble and total calcium and oxalic acid sequentially extracted in solvents of increasing strength (Water, 2N Acetic acid, 5% Perchloric acid or 2N Hydrochloric acid) or directly extracted (in the same solvents) from the foliage of 10 different tree species collected in 2010. (Supplemental information includes data on other elements and organic acids analyzed from the same foliar extracts.)

2. \Data\Minocha_CaOX_Method_data_Exp2.csv: CSV file containing data from repeated experiment for the comparative estimations of soluble and total calcium and oxalic acid sequentially extracted in solvents of increasing strength (Water, 2N Acetic acid, 5% Perchloric acid) or directly extracted (in the same solvents) from the foliage of 4 different tree species collected in 2014. (Supplemental information includes data on other elements and organic acids analyzed from the same foliar extracts.)

Variables in both data files include:

ID = "In-house" sample identification (Tree type-replicate number)

tree = Assigned tree number for identification (1-4)

Replicate = Assigned replicate identification (1-10, a-b)

species = Species of tree that foliage was collected from and needle age-class (Red spruce [Picea rubens], Eastern hemlock [Tsuga canadensis], White pine [Pinus strobus], Norway spruce [Picea abies], Balsam fir [Abies balsamea], American beech [Fagus grandifolia], Red oak [Quercus rubra], Trembling aspen [Populus tremuloides], Yellow birch [Betula alleghaniensis], Black oak [Quercus velutina], Sugar maple [Acer saccharum], CY = current year growth, PY = one-year growth)

Location = Sites where foliar samples were collected (Hedgehog Mountain, Albany, NH, USA [43.9742, -71.3672]; University of New Hampshire-Woodman Horticultural Farm, Durham, NH, USA [43.1477, -70.9407]; the grounds of the USDA Forest Service, Louis C. Wyman Forestry Sciences Laboratory, Durham, NH, USA [43.1423, -70.9492]; University of New Hampshire-Kingman Farm, Madbury, NH, USA [43.1726, -70.9294]; University of New Hampshire-East Foss Farm, Durham, NH, USA [43.1189, -70.9368])

experiment = There were 2 parts to this research (Experiment "1" was done with foliage of different species collected from June through October, 2010. Experiment "2" was a replicate of the sequential extractions and direct extraction in PCA from experiment 1, and was done with foliage from different species all collected in June, 2014.)

date collected = Date foliage samples were collected by month-year (June to October, 2010 and June, 2014)

Ca-Total = Total Calcium (Ca) in foliage extracted in 15.8N Nitric acid by Microwave Digestion measured by Inductively Coupled Plasma Optical Emission Spectrophotometer (ICP-OES), µmol g⁻¹ calc FW

Ca-Seq1-DDW = The portion of total Ca in foliage that is solubilized by 3 freeze/thaw (F/T) cycles in double deionized water (DDW) in the first of a sequence of extractions in three solvents of increasing acidic strength (1st = DDW, 2nd = 2N Acetic acid, 3rd = either 2N Hydrochloric acid OR 5% (0.83N) Perchloric acid) measured by ICP-OES, µmol g⁻¹ FW

Ox-Seq1-DDW = The portion of Oxalic acid (Ox) in foliage that is solubilized by 3 F/T cycles in double deionized water in the first of a sequence of extractions in three solvents of increasing acidic strength (1st = DDW, 2nd = 2N Acetic acid, 3rd = either 2N Hydrochloric acid OR 5% (0.83N) Perchloric acid) measured by High-Performance Liquid Chromatograph, fluorescence (HPLC), µmol g⁻¹ FW

Ca-Seq2-Acetic = The portion of total Ca in foliage that is solubilized by 3 F/T cycles in 2N Acetic acid (Acetic) in the second of a sequence of extractions in three solvents of increasing acidic strength by ICP-OES, µmol g⁻¹ FW

Ox-Seq2-Acetic = The portion of Ox in foliage that is solubilized by 3 F/T cycles in 2N Acetic acid in the second of a sequence of extractions in three solvents of increasing acidic strength by HPLC, µmol g⁻¹ FW

Ca-Seq3A-PCA = The portion of total Ca in foliage that is solubilized by 3 F/T cycles in 5% (0.83N) Perchloric acid (PCA) in the third/final extraction in a sequence of three solvents of increasing acidic strength by ICP-OES, µmol g⁻¹ FW

Ox-Seq3A-PCA = The portion of Ox in foliage that is solubilized by 3 F/T cycles in 5% (0.83N) Perchloric acid in the third/final extraction in a sequence of three solvents of increasing acidic strength by HPLC, µmol g⁻¹ FW

Ca-Seq3B-HCl = The portion of total Ca in foliage that is solubilized by 3 F/T cycles in 2N Hydrochloric acid (HCl) in the third/final extraction in a sequence of three solvents of increasing acidic strength by ICP-OES, µmol g⁻¹ FW

Ox-Seq3B-HCl = The portion of Ox in foliage that is solubilized by 3 F/T cycles in 2N Hydrochloric acid in the third/final extraction in a sequence of three solvents of increasing acidic strength by HPLC, µmol g⁻¹ FW

Ca-Direct-DDW = The portion of total Ca in foliage that is directly solubilized by 3 F/T cycles in DDW by ICP-OES, µmol g⁻¹ FW

Ox-Direct-DDW = Oxalic acid in foliage that is directly solubilized by 3 F/T cycles in DDW by HPLC, µmol g⁻¹ FW

Ca-Direct-Acetic = The portion of total Ca in foliage that is directly solubilized by 3 F/T cycles in 2N Acetic acid by ICP-OES, µmol g⁻¹ FW

Ox-Direct-Acetic = Oxalic acid in foliage that is directly solubilized by 3 F/T cycles in 2N Acetic acid by HPLC, µmol g⁻¹ FW

Ca-Direct-PCA = The portion of total Ca in foliage that is directly solubilized by 3 F/T cycles in 5% PCA by ICP-OES, µmol g⁻¹ FW

Ox-Direct-PCA = Oxalic acid in foliage that is directly solubilized by 3 F/T cycles in 5% PCA by HPLC, µmol g⁻¹ FW

Ca-Direct-HCl = The portion of total Ca in foliage that is directly solubilized by 3 F/T cycles in 2N HCl by ICP-OES, µmol g⁻¹ FW

Ox-Direct-HCl = Oxalic acid in foliage that is directly solubilized by 3 F/T cycles in 2N HCl by HPLC, µmol g⁻¹ FW

Mg-Seq1-DDW = The portion of total Magnesium (Mg) in foliage that is solubilized by 3 freeze/thaw (F/T) cycles in double deionized water (DDW) in the first of a sequence of extractions in three solvents of increasing acidic strength measured by ICP-OES, µmol g⁻¹ FW

Mg-Direct-DDW = The portion of total Mg in foliage that is directly solubilized by 3 F/T cycles in DDW by ICP-OES, µmol g⁻¹ FW

Mg-Seq2-Acetic = The portion of total Mg in foliage that is solubilized by 3 F/T cycles in 2N Acetic acid in the second of a sequence of extractions in three solvents of increasing acidic strength by ICP-OES, µmol g⁻¹ FW

Mg-Direct-Acetic = The portion of total Mg in foliage that is directly solubilized by 3 F/T cycles in 2N Acetic acid by ICP-OES, µmol g⁻¹ FW

Mg-Seq3A-PCA = The portion of total Mg in foliage that is solubilized by 3 F/T cycles in 5% Perchloric acid in the third/final extraction in a sequence of three solvents of increasing acidic strength by ICP-OES, µmol g⁻¹ FW

Mg-DirectPCA = The portion of total Mg in foliage that is directly solubilized by 3 F/T cycles in 5% PCA by ICP-OES, µmol g⁻¹ FW

Mg-Seq3B-HCl = The portion of total Mg in foliage that is solubilized by 3 F/T cycles in 2N Hydrochloric acid in the third/final extraction in a sequence of three solvents of increasing acidic strength by ICP-OES, µmol g⁻¹ FW

Mg-Direct-HCl = The portion of total Mg in foliage that is directly solubilized by 3 F/T cycles in 2N HCl by ICP-OES, µmol g⁻¹ FW

Mg-Total = Total Mg in foliage extracted in 15.8N Nitric acid by Microwave Digestion measured by ICP-OES, µmol g⁻¹ calc FW

K-Seq1-DDW = The portion of total Potassium (K) in foliage that is solubilized by 3 freeze/thaw (F/T) cycles in double deionized water (DDW) in the first of a sequence of extractions in three solvents of increasing acidic strength measured by ICP-OES, µmol g⁻¹ FW

K-Direct-DDW = The portion of total K in foliage that is directly solubilized by 3 F/T cycles in DDW by ICP-OES, µmol g⁻¹ FW

K-Seq2-Acetic = The portion of total K in foliage that is solubilized by 3 F/T cycles in 2N Acetic acid in the second of a sequence of extractions in three solvents of increasing acidic strength by ICP-OES, µmol g⁻¹ FW

K-Direct-Acetic = The portion of total K in foliage that is directly solubilized by 3 F/T cycles in 2N Acetic acid by ICP-OES, µmol g⁻¹ FW

K-Seq3A-PCA = The portion of total K in foliage that is solubilized by 3 F/T cycles in 5% Perchloric acid in the third/final extraction in a sequence of three solvents of increasing acidic strength by ICP-OES, µmol g⁻¹ FW

K-DirectPCA = The portion of total K in foliage that is directly solubilized by 3 F/T cycles in 5% PCA by ICP-OES, µmol g⁻¹ FW

K-Seq3B-HCl = The portion of total K in foliage that is solubilized by 3 F/T cycles in 2N Hydrochloric acid in the third/final extraction in a sequence of three solvents of increasing acidic strength by ICP-OES, µmol g⁻¹ FW

K-Direct-HCl = The portion of total K in foliage that is directly solubilized by 3 F/T cycles in 2N HCl by ICP-OES, µmol g⁻¹ FW

K-Total = Total K in foliage extracted in 15.8N Nitric acid by Microwave Digestion measured by ICP-OES, µmol g⁻¹ calc FW

Mn-Seq1-DDW = The portion of total Manganese (Mn) in foliage that is solubilized by 3 freeze/thaw (F/T) cycles in double deionized water (DDW) in the first of a sequence of extractions in three solvents of increasing acidic strength measured by ICP-OES, µmol g⁻¹ FW

Mn-Direct-DDW = The portion of total Mn in foliage that is directly solubilized by 3 F/T cycles in DDW by ICP-OES, µmol g⁻¹ FW

Mn-Seq2-Acetic = The portion of total Mn in foliage that is solubilized by 3 F/T cycles in 2N Acetic acid in the second of a sequence of extractions in three solvents of increasing acidic strength by ICP-OES, µmol g⁻¹ FW

Mn-Direct-Acetic = The portion of total Mn in foliage that is directly solubilized by 3 F/T cycles in 2N Acetic acid by ICP-OES, µmol g⁻¹ FW

Mn-Seq3A-PCA = The portion of total Mn in foliage that is solubilized by 3 F/T cycles in 5% Perchloric acid in the third/final extraction in a sequence of three solvents of increasing acidic strength by ICP-OES, µmol g⁻¹ FW

Mn-Direct-PCA = The portion of total Mn in foliage that is directly solubilized by 3 F/T cycles in 5% PCA by ICP-OES, µmol g⁻¹ FW

Mn-Seq3B-HCl = The portion of total Mn in foliage that is solubilized by 3 F/T cycles in 2N Hydrochloric acid in the third/final extraction in a sequence of three solvents of increasing acidic strength by ICP-OES, µmol g⁻¹ FW

Mn-Direct-HCl = The portion of total Mn in foliage that is directly solubilized by 3 F/T cycles in 2N HCl by ICP-OES, µmol g⁻¹ FW

Mn-Total = Total Mn in foliage extracted in 15.8N Nitric acid by Microwave Digestion measured by ICP-OES, µmol g⁻¹ calc FW

P-Seq1-DDW = The portion of total Phosphorous (P) in foliage that is solubilized by 3 freeze/thaw (F/T) cycles in double deionized water (DDW) in the first of a sequence of extractions in three solvents of increasing acidic strength measured by ICP-OES, µmol g⁻¹ FW

P-Direct-DDW = The portion of total P in foliage that is directly solubilized by 3 F/T cycles in DDW by ICP-OES, µmol g⁻¹ FW

P-Seq2-Acetic = The portion of total P in foliage that is solubilized by 3 F/T cycles in 2N Acetic acid in the second of a sequence of extractions in three solvents of increasing acidic strength by ICP-OES, µmol g⁻¹ FW

P-Direct-Acetic = The portion of total P in foliage that is directly solubilized by 3 F/T cycles in 2N Acetic acid by ICP-OES, µmol g⁻¹ FW

P-Seq3A-PCA = The portion of total P in foliage that is solubilized by 3 F/T cycles in 5% Perchloric acid in the third/final extraction in a sequence of three solvents of increasing acidic strength by ICP-OES, µmol g⁻¹ FW

P-DirectPCA = The portion of total P in foliage that is directly solubilized by 3 F/T cycles in 5% PCA by ICP-OES, µmol g⁻¹ FW

P-Seq3B-HCl = The portion of total P in foliage that is solubilized by 3 F/T cycles in 2N Hydrochloric acid in the third/final extraction in a sequence of three solvents of increasing acidic strength by ICP-OES, µmol g⁻¹ FW

P-Direct-HCl = The portion of total P in foliage that is directly solubilized by 3 F/T cycles in 2N HCl by ICP-OES, µmol g⁻¹ FW

P-Total = Total P in foliage extracted in 15.8N Nitric acid by Microwave Digestion measured by ICP-OES, µmol g⁻¹ calc FW

Al-Seq1-DDW = The portion of total Aluminum (Al) in foliage that is solubilized by 3 freeze/thaw (F/T) cycles in double deionized water (DDW) in the first of a sequence of extractions in three solvents of increasing acidic strength measured by ICP-OES, µmol g⁻¹ FW

Al-Direct-DDW = The portion of total Al in foliage that is directly solubilized by 3 F/T cycles in DDW by ICP-OES, µmol g⁻¹ FW

Al-Seq2-Acetic = The portion of total Al in foliage that is solubilized by 3 F/T cycles in 2N Acetic acid in the second of a sequence of extractions in three solvents of increasing acidic strength by ICP-OES, µmol g⁻¹ FW

Al-Direct-Acetic = The portion of total Al in foliage that is directly solubilized by 3 F/T cycles in 2N Acetic acid by ICP-OES, µmol g⁻¹ FW

Al-Seq3A-PCA = The portion of total Al in foliage that is solubilized by 3 F/T cycles in 5% Perchloric acid in the third/final extraction in a sequence of three solvents of increasing acidic strength by ICP-OES, µmol g⁻¹ FW

Al-DirectPCA = The portion of total Al in foliage that is directly solubilized by 3 F/T cycles in 5% PCA by ICP-OES, µmol g⁻¹ FW

Al-Seq3B-HCl = The portion of total Al in foliage that is solubilized by 3 F/T cycles in 2N Hydrochloric acid in the third/final extraction in a sequence of three solvents of increasing acidic strength by ICP-OES, µmol g⁻¹ FW

Al-Direct-HCl = The portion of total Al in foliage that is directly solubilized by 3 F/T cycles in 2N HCl by ICP-OES, µmol g⁻¹ FW

Al-Total = Total Al in foliage extracted in 15.8N Nitric acid by Microwave Digestion measured by ICP-OES, µmol g⁻¹ calc FW

Fe-Seq1-DDW = The portion of total Iron (Fe) in foliage that is solubilized by 3 freeze/thaw (F/T) cycles in double deionized water (DDW) in the first of a sequence of extractions in three solvents of increasing acidic strength measured by ICP-OES, µmol g⁻¹ FW

Fe-Direct-DDW = The portion of total Fe in foliage that is directly solubilized by 3 F/T cycles in DDW by ICP-OES, µmol g⁻¹ FW

Fe-Seq2-Acetic = The portion of total Fe in foliage that is solubilized by 3 F/T cycles in 2N Acetic acid in the second of a sequence of extractions in three solvents of increasing acidic strength by ICP-OES, µmol g⁻¹ FW

Fe-Direct-Acetic = The portion of total Fe in foliage that is directly solubilized by 3 F/T cycles in 2N Acetic acid by ICP-OES, µmol g⁻¹ FW

Fe-Seq3A-PCA = The portion of total Fe in foliage that is solubilized by 3 F/T cycles in 5% Perchloric acid in the third/final extraction in a sequence of three solvents of increasing acidic strength by ICP-OES, µmol g⁻¹ FW

Fe-DirectPCA = The portion of total Fe in foliage that is directly solubilized by 3 F/T cycles in 5% PCA by ICP-OES, µmol g⁻¹ FW

Fe-Seq3B-HCl = The portion of total Fe in foliage that is solubilized by 3 F/T cycles in 2N Hydrochloric acid in the third/final extraction in a sequence of three solvents of increasing acidic strength by ICP-OES, µmol g⁻¹ FW

Fe-Direct-HCl = The portion of total Fe in foliage that is directly solubilized by 3 F/T cycles in 2N HCl by ICP-OES, µmol g⁻¹ FW

Fe-Total = Total Fe in foliage extracted in 15.8N Nitric acid by Microwave Digestion measured by ICP-OES, µmol g⁻¹ calc FW

Zn-Seq1-DDW = The portion of total Zinc (Zn) in foliage that is solubilized by 3 freeze/thaw (F/T) cycles in double deionized water (DDW) in the first of a sequence of extractions in three solvents of increasing acidic strength measured by ICP-OES, µmol g⁻¹ FW

Zn-Direct-DDW = The portion of total Zn in foliage that is directly solubilized by 3 F/T cycles in DDW by ICP-OES, µmol g⁻¹ FW

Zn-Seq2-Acetic = The portion of total Zn in foliage that is solubilized by 3 F/T cycles in 2N Acetic acid in the second of a sequence of extractions in three solvents of increasing acidic strength by ICP-OES, µmol g⁻¹ FW

Zn-Direct-Acetic = The portion of total Zn in foliage that is directly solubilized by 3 F/T cycles in 2N Acetic acid by ICP-OES, µmol g⁻¹ FW

Zn-Seq3A-PCA = The portion of total Zn in foliage that is solubilized by 3 F/T cycles in 5% Perchloric acid in the third/final extraction in a sequence of three solvents of increasing acidic strength by ICP-OES, µmol g⁻¹ FW

Zn-DirectPCA = The portion of total Zn in foliage that is directly solubilized by 3 F/T cycles in 5% PCA by ICP-OES, µmol g⁻¹ FW

Zn-Seq3B-HCl = The portion of total Zn in foliage that is solubilized by 3 F/T cycles in 2N Hydrochloric acid (HCl) in the third/final extraction in a sequence of three solvents of increasing acidic strength by ICP-OES, µmol g⁻¹ FW

Zn-Direct-HCl = The portion of total Zn in foliage that is directly solubilized by 3 F/T cycles in 2N HCl by ICP-OES, µmol g⁻¹ FW

Zn-Total = Total Zn in foliage extracted in 15.8N Nitric acid by Microwave Digestion measured by ICP-OES, µmol g⁻¹ calc FW

Mal-Seq1-DDW = The portion of Malic acid (Mal) in foliage that is solubilized by 3 F/T cycles in double deionized water in the first of a sequence of extractions in three solvents of increasing acidic strength measured by HPLC, µmol g⁻¹ FW

Mal-Direct-DDW = Malic acid in foliage that is directly solubilized by 3 F/T cycles in DDW by HPLC, µmol g⁻¹ FW

Mal-Seq2-Acetic = The portion of Mal in foliage that is solubilized by 3 F/T cycles in 2N Acetic acid in the second of a sequence of extractions in three solvents of increasing acidic strength by HPLC, µmol g⁻¹ FW

Mal-Direct-Acetic = Malic acid in foliage that is directly solubilized by 3 F/T cycles in 2N Acetic acid by HPLC, µmol g⁻¹ FW

Mal-Seq3A-PCA = The portion of Mal in foliage that is solubilized by 3 F/T cycles in 5% (0.83N) Perchloric acid in the third/final extraction in a sequence of three solvents of increasing acidic strength by HPLC, µmol g⁻¹ FW

Mal-Direct-PCA = Malic acid in foliage that is directly solubilized by 3 F/T cycles in 5% PCA by HPLC, µmol g⁻¹ FW

Mal-Seq3B-HCl = The portion of Mal in foliage that is solubilized by 3 F/T cycles in 2N Hydrochloric acid in the third/final extraction in a sequence of three solvents of increasing acidic strength by HPLC, µmol g⁻¹ FW

Mal-Direct-HCl = Malic acid in foliage that is directly solubilized by 3 F/T cycles in 2N HCl by HPLC, µmol g⁻¹ FW

Asc-Seq1-DDW = The portion of Ascorbic acid (Asc) in foliage that is solubilized by 3 F/T cycles in double deionized water in the first of a sequence of extractions in three solvents of increasing acidic strength measured by HPLC, µmol g⁻¹ FW

Asc-Direct-DDW = Ascorbic acid in foliage that is directly solubilized by 3 F/T cycles in DDW by HPLC, µmol g⁻¹ FW

Asc-Seq2-Acetic = The portion of Asc in foliage that is solubilized by 3 F/T cycles in 2N Acetic acid in the second of a sequence of extractions in three solvents of increasing acidic strength by HPLC, µmol g⁻¹ FW

Asc-Direct-Acetic = Ascorbic acid in foliage that is directly solubilized by 3 F/T cycles in 2N Acetic acid by HPLC, µmol g⁻¹ FW

Asc-Seq3A-PCA = The portion of Asc in foliage that is solubilized by 3 F/T cycles in 5% (0.83N) Perchloric acid in the third/final extraction in a sequence of three solvents of increasing acidic strength by HPLC, µmol g⁻¹ FW

Asc-Direct-PCA = Ascorbic acid in foliage that is directly solubilized by 3 F/T cycles in 5% PCA by HPLC, µmol g⁻¹ FW

Asc-Seq3B-HCl = The portion of Asc in foliage that is solubilized by 3 F/T cycles in 2N Hydrochloric acid in the third/final extraction in a sequence of three solvents of increasing acidic strength by HPLC, µmol g⁻¹ FW

Asc-Direct-HCl = Ascorbic acid in foliage that is directly solubilized by 3 F/T cycles in 2N HCl by HPLC, µmol g⁻¹ FW

α-Keto-Seq1-DDW = The portion of alpha (α)-Ketoglutaric acid (α-Keto) in foliage that is solubilized by 3 F/T cycles in double deionized water in the first of a sequence of extractions in three solvents of increasing acidic strength measured by HPLC, µmol g⁻¹ FW

α-Keto-Direct-DDW = α-Ketoglutaric acid in foliage that is directly solubilized by 3 F/T cycles in DDW by HPLC, µmol g⁻¹ FW

α-Keto-Seq2-Acetic = The portion of α-Keto in foliage that is solubilized by 3 F/T cycles in 2N Acetic acid in the second of a sequence of extractions in three solvents of increasing acidic strength by HPLC, µmol g⁻¹ FW

α-Keto-Direct-Acetic = α-Ketoglutaric acid in foliage that is directly solubilized by 3 F/T cycles in 2N Acetic acid by HPLC, µmol g⁻¹ FW

α-Keto-Seq3A-PCA = The portion of α-Keto in foliage that is solubilized by 3 F/T cycles in 5% (0.83N) Perchloric acid in the third/final extraction in a sequence of three solvents of increasing acidic strength by HPLC, µmol g⁻¹ FW

α-Keto-Direct-PCA = α-Ketoglutaric acid in foliage that is directly solubilized by 3 F/T cycles in 5% PCA by HPLC, µmol g⁻¹ FW

α-Keto-Seq3B-HCl = The portion of α-Keto in foliage that is solubilized by 3 F/T cycles in 2N Hydrochloric acid in the third/final extraction in a sequence of three solvents of increasing acidic strength by HPLC, µmol g⁻¹ FW

α-Keto-Direct-HCl = α-Ketoglutaric acid in foliage that is directly solubilized by 3 F/T cycles in 2N HCl by HPLC, µmol g⁻¹ FW

Cit-Seq1-DDW = The portion of Citric acid (Cit) in foliage that is solubilized by 3 F/T cycles in double deionized water in the first of a sequence of extractions in three solvents of increasing acidic strength measured by HPLC, µmol g⁻¹ FW

Cit-Direct-DDW = Citric acid in foliage that is directly solubilized by 3 F/T cycles in DDW by HPLC, µmol g⁻¹ FW

Cit-Seq2-Acetic = The portion of Cit in foliage that is solubilized by 3 F/T cycles in 2N Acetic acid in the second of a sequence of extractions in three solvents of increasing acidic strength by HPLC, µmol g⁻¹ FW

Cit-Direct-Acetic = Citric acid in foliage that is directly solubilized by 3 F/T cycles in 2N Acetic acid by HPLC, µmol g⁻¹ FW

Cit-Seq3A-PCA = The portion of Cit in foliage that is solubilized by 3 F/T cycles in 5% (0.83N) Perchloric acid in the third/final extraction in a sequence of three solvents of increasing acidic strength by HPLC, µmol g⁻¹ FW

Cit-Direct-PCA = Citric acid in foliage that is directly solubilized by 3 F/T cycles in 5% PCA by HPLC, µmol g⁻¹ FW

Cit-Seq3B-HCl = The portion of Cit in foliage that is solubilized by 3 F/T cycles in 2N Hydrochloric acid in the third/final extraction in a sequence of three solvents of increasing acidic strength by HPLC, µmol g⁻¹ FW

Cit-Direct-HCl = Citric acid in foliage that is directly solubilized by 3 F/T cycles in 2N HCl by HPLC, µmol g⁻¹ FW

Succ-Seq1-DDW = The portion of Succinic acid (Succ) in foliage that is solubilized by 3 F/T cycles in double deionized water in the first of a sequence of extractions in three solvents of increasing acidic strength measured by HPLC, µmol g⁻¹ FW

Succ-Direct-DDW = Succinic acid in foliage that is directly solubilized by 3 F/T cycles in DDW by HPLC, µmol g⁻¹ FW

Succ-Seq2-Acetic = The portion of Succ in foliage that is solubilized by 3 F/T cycles in 2N Acetic acid in the second of a sequence of extractions in three solvents of increasing acidic strength by HPLC, µmol g⁻¹ FW

Succ-Direct-Acetic = Succinic acid in foliage that is directly solubilized by 3 F/T cycles in 2N Acetic acid by HPLC, µmol g⁻¹ FW

Succ-Seq3A-PCA = The portion of Succ in foliage that is solubilized by 3 F/T cycles in 5% (0.83N) Perchloric acid in the third/final extraction in a sequence of three solvents of increasing acidic strength by HPLC, µmol g⁻¹ FW

Succ-Direct-PCA = Succinic acid in foliage that is directly solubilized by 3 F/T cycles in 5% PCA by HPLC, µmol g⁻¹ FW

Succ-Seq3B-HCl = The portion of Succ in foliage that is solubilized by 3 F/T cycles in 2N Hydrochloric acid in the third/final extraction in a sequence of three solvents of increasing acidic strength by HPLC, µmol g⁻¹ FW

Succ-Direct-HCl = Succinic acid in foliage that is directly solubilized by 3 F/T cycles in 2N HCl by HPLC, µmol g⁻¹ FW

Entity_and_Attribute_Detail_Citation:

Minocha, Rakesh; Chamberlain, Bradley; Long, Stephanie; Turlapati, Swathi A.; Quigley, Gloria. 2015. Extraction and estimation of the quantity of calcium oxalate crystals in the foliage of conifer and hardwood trees. Tree Physiology, 35(5): 574-580. https://doi.org/10.1093/treephys/tpv031

Minocha, Rakesh; Long, Stephanie; Thangavel, Palaniswamy; Minocha, Subhash C.; Eagar, Christopher; Driscoll, Charles T. 2010. Elevation dependent sensitivity of northern hardwoods to Ca addition at Hubbard Brook Experimental Forest, NH, USA. Forest Ecology and Management 260(12): 2115-2124. https://doi.org/10.1016/j.foreco.2010.09.002

Metadata_Reference_Information:

Metadata_Date: 20220617

Metadata_Contact:

Contact_Information:

Contact_Organization_Primary:

Contact_Organization: USDA Forest Service, Northern Research Station

Contact_Person: Rakesh Minocha

Contact_Position: Plant Physiologist

Contact_Address:

Address_Type: mailing and physical

Address: 271 Mast Road

City: Durham

State_or_Province: NH

Postal_Code: 03824

Country: USA

Contact_Voice_Telephone: 603-868-7622

Contact_Electronic_Mail_Address: rakesh.minocha@usda.gov

Contact_Information:

Contact_Organization_Primary:

Contact_Organization: USDA Forest Service, Northern Research Station

Contact_Person: Stephanie Long

Contact_Position: Biological Technician

Contact_Address:

Address_Type: mailing and physical

Address: 271 Mast Road

City: Durham

State_or_Province: NH

Postal_Code: 03824

Country: USA

Contact_Voice_Telephone: 603-868-7623

Contact_Electronic_Mail_Address: stephanie.long2@usda.gov

Metadata_Standard_Name: FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata

Metadata_Standard_Version: FGDC-STD-001.1-1999