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Keyword: DNA

Detecting rare species using environmental DNA

Projects Posted on: September 23, 2015
External DNA released by animals in aquatic environments, called environmental DNA (eDNA), can be used to determine whether a species is present without actually capturing or seeing an individual. Because of its greater efficiency and reduced cost, eDNA sampling may revolutionize the monitoring and assessment of freshwater species.

DNA barcoding at riverscape scales

Documents and Media Posted on: December 03, 2014
Projections of a rapidly changing climate and increasing human population have led to calls for broadscale biodiversity assessments that can serve as benchmarks for identifying ecological change. Genetic tools have been used for such assessments for decades, but spatial sampling considerations have been overlooked.Document Type: Briefing Papers

Development of PCR-RFP and DNA barcoding plastic markers for yellow toadflax and Dalmatian toadflax

Publications Posted on: May 06, 2014
Yellow toadflax and Dalmatian toadflax are problematic invasive plant species in North America. Yellow toadflax was introduced multiple times to the United States from Europe, beginning in the late 1600s. Dalmatian toadflax has similarly been repeatedly introduced to the United States, starting in 1874. Both species are known to inhabit disturbed areas, competing for limiting resources with native plant species.

From diagnostics to metagenomics: Applications of DNA-based tools in forest pathology

Publications Posted on: October 24, 2013
Advances in molecular technology provide an accessible set of tools to 1) help forest pathologists detect, identify, and monitor forest pathogens, 2) examine the evolutionary relationships and global distributions of forest pathogens and their hosts, 3) assess the diversity and structure of host and pathogen populations, and 4) evaluate the structure and function of genes, as well as their levels of expression, within species and within commun

DNA-based approaches to identify forest fungi in Pacific Islands: A pilot study

Publications Posted on: October 24, 2013
DNA-based diagnostics have been successfully used to characterize diverse forest fungi (e.g., Hoff et al. 2004, Kim et al. 2006, Glaeser & Lindner 2011). DNA sequencing of the internal transcribed spacer (ITS) and large subunit (LSU) regions of nuclear ribosomal DNA (rDNA) has proved especially useful (Sonnenberg et al. 2007, Seifert 2009, Schoch et al. 2012) for identification.

Historical and contemporary DNA indicate fisher decline and isolation occurred prior to the European settlement of California

Publications Posted on: February 05, 2013
Establishing if species contractions were the result of natural phenomena or human induced landscape changes is essential for managing natural populations. Fishers (Martes pennanti) in California occur in two geographically and genetically isolated populations in the northwestern mountains and southern Sierra Nevada.

Developing a prediction model for Armillaria solidipes in Arizona

Publications Posted on: November 09, 2012
In 2010, a collaborative project was started to determine the distribution of Armillaria solidipes (= A. ostoyae) in Arizona. The methods and preliminary accomplishments of the 2010 and 2011 (ongoing) field surveys/collections are summarized. During the next phase of this project, surveys will be completed and remaining Armillaria isolates will be identified using DNA-based methods.

DNA-based identification of Armillaria isolates from peach orchards in Mexico state

Publications Posted on: November 09, 2012
A collaborative project between the Programa de Fitopatología, Colegio de Postgraduados, Texcoco, Estado de Mexico and the USDA Forest Service - RMRS, Moscow Forest Pathology Laboratory has begun this year (2011) to assess which species of Armillaria are causing widespread and severe damage to the peach orchards from México state, Mexico.

Weathered antlers as a source of DNA

Publications Posted on: August 15, 2012
We tested antlers of Coues white-tailed (Odocoileus virginianus couesi) and mule deer (O. hemionus) in various stages of natural decomposition to determine the degree of weathering that cast antlers could endure and still yield usable DNA. Based on physical characteristics, we partitioned antlers into 7 weathering categories ranging from freshly cast (class 1) to having been exposed to weathering for 8 years (class 7).

Integrating motion-detection cameras and hair snags for wolverine identification

Publications Posted on: March 20, 2012
We developed an integrated system for photographing a wolverine's (Gulo gulo) ventral pattern while concurrently collecting hair for microsatellite DNA genotyping.