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Keyword: environmental DNA (eDNA)

Environmental DNA sampling of small-bodied minnows: Performance relative to location, species, and traditional sampling

Publications Posted on: October 02, 2019
We performed experiments in southwestern USA streams to evaluate the efficacy of environmental DNA (eDNA) sampling for two rare small-bodied minnows: Spikedace Meda fulgida and Loach Minnow Rhinichthys cobitis. We collected eDNA by filtering 5-L samples and compared detection sensitivity of eDNA assays to traditional sampling methods (electrofishing and seining) by using both techniques at 33 sites in seven streams.

Rare carnivore detections from environmental DNA in snow

Media Gallery Posted on: September 05, 2019
A new project showed that animal footprints in snow contain enough DNA for species identification, even when the snow was many months old. The study extracted DNA from snow samples collected within animal tracks as well as areas where the animal had been photographed months earlier. Newly developed genetic assays were applied and positively detected the DNA of each species, performing nearly flawlessly on samples previously considered too poor to provide usable DNA. This method could revolutionize winter surveys of rare species by greatly reducing or eliminating misidentifications and missed detections.

Rare carnivore detections from environmental DNA in snow

Science Spotlights Posted on: September 05, 2019
A new project showed that animal footprints in snow contain enough DNA for species identification, even when the snow was many months old. The study extracted DNA from snow samples collected within animal tracks as well as areas where the animal had been photographed months earlier. Newly developed genetic assays were applied and positively detected the DNA of each species, performing nearly flawlessly on samples previously considered too poor to provide usable DNA. This method could revolutionize winter surveys of rare species by greatly reducing or eliminating misidentifications and missed detections.

Fine-scale environmental DNA sampling reveals climate-mediated interactions between native and invasive trout species

Publications Posted on: November 29, 2018
It is widely recognized that biotic interactions may act as important mediators of species responses to climate change. However, collecting the abiotic and biotic covariates at the resolution and extent needed to reveal these interactions from species distribution models is often prohibitively expensive and labor-intensive.

DNA barcoding at riverscape scales

Science Spotlights Posted on: April 29, 2015
Research conducted by RMRS scientists demonstrates how intensive sampling efforts across a large geographical scale can influence identification of taxonomic groups among the fishes of the genus Cottus in the northern Rocky Mountains. Researchers used specific sequences from mitochondrial DNA regions and phylogenetic analyses techniques as indicators of biodiversity and to identify unique species.

Detecting rare species using environmental DNA

Documents and Media Posted on: December 03, 2014
Animals in aquatic environments—such as fish, amphibians, crayfish, and mussels—release DNA into the water via their feces, urine, and skin. This external DNA is called environmental DNA (eDNA). By filtering water samples and analyzing them for eDNA, one can determine whether a species is present without actually capturing or seeing an individual. Different species can be identified by using genetic markers that are unique to them. Document Type: Briefing Papers

Robust detection of rare species using environmental DNA: The importance of primer specificity

Publications Posted on: April 29, 2013
Environmental DNA (eDNA) is being rapidly adopted as a tool to detect rare animals. Quantitative PCR (qPCR) using probebased chemistries may represent a particularly powerful tool because of the method's sensitivity, specificity, and potential to quantify target DNA. However, there has been little work understanding the performance of these assays in the presence of closely related, sympatric taxa.