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Keyword: qPCR

Parallel, targeted analysis of environmental samples via high-throughput quantitative PCR

Publications Posted on: May 10, 2020
When analyzing environmental samples for DNA from multiple taxa, researchers must usually decide between iterative analyses with single-taxon assays - which are reliable and sensitive, but also laborious to apply - and approaches such as metabarcoding that can simultaneously target multiple species, but which are less sensitive for detection across taxa.

Using environmental DNA methods to improve winter surveys for rare carnivores: DNA from snow and improved noninvasive techniques

Publications Posted on: November 29, 2018
The management of rare species is a conservation priority worldwide, but this task is made difficult by detection errors in population surveys. Both false positive (misidentification) and false negative (missed detection) errors are prevalent in surveys for rare species and can affect resulting inferences about their population status or distribution.

Inferring presence of the western toad (Anaxyrus boreas) species complex using environmental DNA

Publications Posted on: September 17, 2018
Western toads (species complex comprised of Anaxyrus boreas, A. canorus, A. exsul, and A. nelsoni) are widely distributed in the western United States but are declining, particularly in the southeastern extent of their range. The subspecies A. b. boreas is listed as a Species of Greatest Conservation Need in New Mexico, Colorado, Utah, and Wyoming.

A non-invasive sampling method for detecting non-native smallmouth bass (Micropterus dolomieu)

Publications Posted on: July 23, 2018
The smallmouth bass (Micropterus dolomieu) is a cool-water fish species native to central North America. Widespread introductions and secondary spread outside of its historical range have led to new recreational fisheries and associated economic benefits in western United States, but have also resulted in a number of ecological impacts to recipient ecosystems, including threats to Pacific salmon.

An environmental DNA assay for detecting Arctic grayling in the upper Missouri River basin, North America

Publications Posted on: September 12, 2016
The upper Missouri River basin in the northwestern US contains disjunct Arctic grayling (Thymallus arcticus) populations of conservation concern. To assist efforts aimed at understanding Artic grayling distribution, we developed a quantitative PCR assay to detect the presence of Arctic grayling DNA in environmental samples.

An environmental DNA marker for detecting nonnative brown trout (Salmo trutta)

Publications Posted on: June 21, 2016
Brown trout (Salmo trutta) are widely introduced in western North America where their presence has led to declines of several native species. To assist conservation efforts aimed at early detection and eradication of this species, we developed a quantitative PCR marker to detect the presence of brown trout DNA in environmental samples.

Detection and range delineation of bull trout using environmental DNA

Science Spotlights Posted on: May 20, 2015
The bull trout is an ESA-listed species that relies on cold stream environments across the Northwest and is expected to decline with climate change. Resource managers from dozens of agencies are charged with maintaining bull trout in thousands of streams, but monitoring this species is difficult. Environmental DNA (eDNA) is much faster, easier, and more sensitive than traditional fish sampling methods and provides an opportunity to better delineate populations of federally threatened species like bull trout.

Distance, flow and PCR inhibition: eDNA dynamics in two headwater streams

Publications Posted on: September 11, 2014
Environmental DNA (eDNA) detection has emerged as a powerful tool for monitoring aquatic organisms, but much remains unknown about the dynamics of aquatic eDNA over a range of environmental conditions. DNA concentrations in streams and rivers will depend not only on the equilibrium between DNA entering the water and DNA leaving the system through degradation, but also on downstream transport.