Landscape genetic studies have focused on terrestrial animals more than any other taxonomic group. This chapter focuses on applications of landscape genetics for understanding connectivity of terrestrial animal populations. It starts with a general introduction covering unique characteristics and challenges of the terrestrial study system. This is followed by an overview of common research questions addressed in terrestrial animals using neutral markers in landscape genetics. These common research questions include detecting and defining barriers, identifying corridors, examining source-sink dynamics, and detecting and predicting animal responses to environmental change. The chapter highlights limitations of current research approaches and presents case studies that have specifically dealt with some of these limitations when testing ecological hypotheses about gene flow in heterogeneous landscapes. It concludes with a discussion on the future directions and current knowledge gaps for terrestrial animal landscape genetic studies.