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Genetic mapping of Pinus flexilis major gene (Cr4) for resistance to white pine blister rust using transcriptome-based SNP genotyping

Posted date: October 04, 2016
Publication Year: 
2016
Authors: Liu, Jun-Jun; Schoettle, Anna W.; Sniezko, Richard A.; Sturrock, Rona N.; Zamany, Arezoo; Williams, Holly; Ha, Amanda; Chan, Danelle; Danchok, Bob; Savin, Douglas P.; Kegley, Angelia
Publication Series: 
Scientific Journal (JRNL)
Source: BMC Genomics. 17: 753.

Abstract

Linkage of DNA markers with phenotypic traits provides essential information to dissect clustered genes with potential phenotypic contributions in a target genome region. Pinus flexilis E. James (limber pine) is a keystone five-needle pine species in mountain-top ecosystems of North America. White pine blister rust (WPBR), caused by a non-native fungal pathogen Cronartium ribicola (J.C. Fisch.), has resulted in mortality in this conifer species and is still spreading through the distribution. The objective of this research was to develop P. flexilis transcriptome-wide single nucleotide polymorphism (SNP) markers using RNA-seq analysis for genetic mapping of the major gene (Cr4) that confers complete resistance to C. ribicola.

Citation

Liu, Jun-Jun; Schoettle, Anna W.; Sniezko, Richard A.; Sturrock, Rona N.; Zamany, Arezoo; Williams, Holly; Ha, Amanda; Chan, Danelle; Danchok, Bob; Savin, Douglas P.; Kegley, Angelia. 2016. Genetic mapping of Pinus flexilis major gene (Cr4) for resistance to white pine blister rust using transcriptome-based SNP genotyping. BMC Genomics. 17: 753.