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Individual identification of Sitka black-tailed deer (Odocoileus hemionus sitkensis) using DNA from fecal pellets

Posted date: March 15, 2012
Publication Year: 
2010
Authors: Brinkman, Todd J.; Person, David K.; Schwartz, Michael K.Pilgrim, Kristine L.; Colson, Kevin E.; Hundertmark, Kris J.
Publication Series: 
Miscellaneous Publication
Source: Conservation Genetics Resources. 2: 115-118.

Abstract

We tested a protocol for extracting DNA from fecal pellets from Sitka black-tailed deer (Odocoileus hemionus sitkensis) and evaluated genotyping performance of previously developed microsatellite markers as well as a suite of new markers designed specifically for this study. We screened 30 microsatellites, and identified 7 (23%) loci including 4 new markers, that fit well into a single multiplex and consistently genotyped deer with low error rates. DNA was extracted from 2,408 fecal-pellet samples. Of those, 1,240 (52%) were genotyped successfully at all 7 markers allowing identification of 634 genetically unique deer. Using DNA from fecal pellets collected in the field was an effective technique for identifying and distinguishing among deer.

Citation

Brinkman, Todd J.; Person, David K.; Schwartz, Michael K.; Pilgrim, Kristine L.; Colson, Kevin E.; Hundertmark, Kris J. 2010. Individual identification of Sitka black-tailed deer (Odocoileus hemionus sitkensis) using DNA from fecal pellets. Conservation Genetics Resources. 2: 115-118.