Hawai'i is a biological hotspot with a variety of climates and habitats. While fungal species diversity has been more extensively studied in Hawai'i than other Pacific Islands (e.g. see Gilbertson et al. 2002), there remain many species unreported in the literature. This project attempted to capture a small portion of Hawai'i's fungal diversity in southeast O'ahu by identifying fruit bodies using molecular techniques. The efficacy of using the internal transcribed spacer (ITS) and large subunit (LSU) regions of the nuclear ribosomal DNA (rDNA) to identify Hawai'ian fungi is discussed.