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Effects of cloning and root-tip size on observations of fungal ITS sequences from Picea glauca rootsAuthor(s): Daniel L. Lindner; Mark T. Banik
Source: Mycologia. 101(1): 157?165.
Publication Series: Scientific Journal (JRNL)
Station: Northern Research Station
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DescriptionTo better understand the effects of cloning on observations of fungal ITS sequences from Picea glauca (white spruce) roots two techniques were compared: (i) direct sequencing of fungal ITS regions from individual root tips without cloning and (ii) cloning and sequencing of fungal ITS regions from individual root tips. Effect of root tip size was investigated by selecting 20 small root tips (SRT, 1.0-2.0 mm long) and 20 large root tips (LRT, 5.0- 6.0 mm long). DNA was isolated from each tip and PCR-amplified with fungal-specific primers.
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CitationLindner, Daniel L.; Banik, Mark T. 2009. Effects of cloning and root-tip size on observations of fungal ITS sequences from Picea glauca roots. Mycologia. 101(1): 157-165.
Keywordsectomycorrhizal fungi, endophyte, environmental samples, mycorrhiza, species identification
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