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    Author(s): Daniel L. LindnerMark T. Banik
    Date: 2009
    Source: Mycologia. 101(1): 157?165.
    Publication Series: Scientific Journal (JRNL)
    Station: Northern Research Station
    PDF: View PDF  (330.4 KB)

    Description

    To better understand the effects of cloning on observations of fungal ITS sequences from Picea glauca (white spruce) roots two techniques were compared: (i) direct sequencing of fungal ITS regions from individual root tips without cloning and (ii) cloning and sequencing of fungal ITS regions from individual root tips. Effect of root tip size was investigated by selecting 20 small root tips (SRT, 1.0-2.0 mm long) and 20 large root tips (LRT, 5.0- 6.0 mm long). DNA was isolated from each tip and PCR-amplified with fungal-specific primers.

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    Citation

    Lindner, Daniel L.; Banik, Mark T. 2009. Effects of cloning and root-tip size on observations of fungal ITS sequences from Picea glauca roots. Mycologia. 101(1): 157-165.

    Keywords

    ectomycorrhizal fungi, endophyte, environmental samples, mycorrhiza, species identification

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