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  1. Enzyme immunoassays for detection of gypsy moth nuclear polyhedrosis virus

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    Author(s): Michael Ma
    Date: 1985
    Source: In: Grimble, David G.; Lewis, Franklin B., coords. Proceedings, Symposium: Microbial control of spruce budworms and gypsy moths; 1984 April 10-12; Windsor Locks, CT. Gen. Tech. Rep. NE-100. Broomall, PA: U.S. Department of Agriculture, Forest Service, Northeastern Forest Experiment Station. 125-131
    Publication Series: General Technical Report (GTR)
    Station: Northeastern Research Station
    PDF: View PDF  (586.62 KB)
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    Description

    Enzyme-linked immunosorbent assays (ELISA) were developed for detecting gypsy moth (Lymantria dispar L.) nuclear polyhedrosis virus (NPV). They were used to detect the presence of NPV in hemoplymph samples collected from infected larvae. The incorporation of hybridoma antibodies with these procedures would make them even more specific for gypsy moth NPV detection. A pilot study of evaluating NPV spray with ELISA is also presented here. Potentially, these immunochemical methods have important applications in an IPM approach toward the control of gypsy moth.

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    Citation

    Ma, Michael. 1985. Enzyme immunoassays for detection of gypsy moth nuclear polyhedrosis virus. In: Grimble, David G.; Lewis, Franklin B., coords. Proceedings, Symposium: Microbial control of spruce budworms and gypsy moths; 1984 April 10-12; Windsor Locks, CT. Gen. Tech. Rep. NE-100. Broomall, PA: U.S. Department of Agriculture, Forest Service, Northeastern Forest Experiment Station. 125-131

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