Skip to Main Content
Influence of regeneration method and tissue source on the frequency of somatic variation in Populus to infection by Septoria musivaAuthor(s): Michael E. Ostry; Ronald L. Hackett; Charles H. Michler; R. Serres; B. McCown
Source: Plant Science. Vol. 97 no. 1.:p. 209-215. (1994)
Publication Series: Scientific Journal (JRNL)
Station: North Central Research Station
PDF: View PDF (1.37 MB)
DescriptionSeptoria leaf spot and canker are serious diseases of many hybrid poplar clones in plantations established for biomass production. Developing resistant clones through breeding is the best long-term strategy to minimize tree damage caused by this disease. Tissue culture and somaclonal selection techniques may reduce the time needed to develop disease resistance in poplars. We used a single source clone of a hybrid Populus to determine the influences of explant tissue source and regeneration method on the frequency of somatic variation in disease resistance. Plants were regenerated adventitiously via shoots and somatic embryos from callus derived from several tissue sources and from axillary buds. The resulting plants expressed somatic variation in disease resistance in different frequencies, except for the plants regenerated from hardwood cuttings. Several regenerated plants from various tissue sources exhibited variant morphological phenotypes, providing further evidence of the instability of this clone when cultured in vitro. Although mutagenic effects of the culture regimes alone cannot be ruled out, results of this study suggest that the explant source and culture method influenced how frequently variant plants were recovered.
- Check the Northern Research Station web site to request a printed copy of this publication.
- Our on-line publications are scanned and captured using Adobe Acrobat.
- During the capture process some typographical errors may occur.
- Please contact Sharon Hobrla, email@example.com if you notice any errors which make this publication unusable.
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationOstry, Michael E.; Hackett, Ronald L.; Michler, Charles H.; Serres, R.; McCown, B. 1994. Influence of regeneration method and tissue source on the frequency of somatic variation in Populus to infection by Septoria musiva. Plant Science. Vol. 97 no. 1.:p. 209-215. (1994)
KeywordsOrganogenesis, Somatic embryogenesis, Protoplasts, Micro-cross sections, Root organ cultures, Disease resistance, Hybrid poplar, Intraclonal variation, Somaclonal variation
- Field performance of Populus expressing somaclonal variation in resistance to Septoria musiva
- Drought effects on leaf abscission and leaf production in Populus clones
- The influence of alternative plant propagation and stand establishment techniques on survival and growth of eastern cottonwood (Populus deltoids Bartr.) clones
XML: View XML