Skip to Main Content
Histochemical and microspectrophotometric analyses of early wound responses of resistant and susceptible Populus tremuloides inoculated with Entoleuca mammata (=Hypoxylon mammatum)Author(s): B. Bucciarelli; Michael E. Ostry; R. G. Fulcher; N. A. Anderson; C. P. Vance
Source: Canadian Journal of Botany. 77(4): 548-555.
Publication Series: Scientific Journal (JRNL)
Station: North Central Research Station
PDF: View PDF (1.15 MB)
DescriptionStem tissue of resistant and susceptible genotypes of Poyulus tremuloides Michx, wounded or woundinoculated with Entoleuca mammata (Wahlenberg: Fr.) J.D. Rogers & Y.M. Ju was prepared for histochemical and microspectrophotometric analysis. Samples were collected over a 96-h period. Parenchyma cell walls associated with the response zone of infected resistant and susceptible genotypes accumulated phenolic substances having lignin-like properties. Features of the lignified zone distinguished resistant from susceptible genotypes. This zone in the resistant genotype was uniformly lignified, while in the susceptible genotype, it was discontinuous. Wound callus developed in the infected resistant but not in the susceptible genotype. In the former, callus developed internal to the lignified zone, contained phenolic substances, and was visible 48 h after inoculation. In the susceptible, callus failed to develop. Wounded tissue of both genotypes displayed no distinguishing response characteristics. Both produced equivalent amounts of callus, accumulated similar levels of lignin-like substances, and deposited it in identical locations. It is concluded that resistant P. tremuloldes limits infection by E. mammat, by two distinct mechanisms: (i) by the development of an intact and localized lignified barrier zone and (ii) by the development of wound callus rich in phenolic substances. The susceptible is ineffective at developing either of these barriers.
- Check the Northern Research Station web site to request a printed copy of this publication.
- Our on-line publications are scanned and captured using Adobe Acrobat.
- During the capture process some typographical errors may occur.
- Please contact Sharon Hobrla, email@example.com if you notice any errors which make this publication unusable.
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationBucciarelli, B.; Ostry, Michael E.; Fulcher, R. G.; Anderson, N. A.; Vance, C. P. 1999. Histochemical and microspectrophotometric analyses of early wound responses of resistant and susceptible Populus tremuloides inoculated with Entoleuca mammata (=Hypoxylon mammatum). Canadian Journal of Botany. 77(4): 548-555.
Keywordsaspen, Hypoxylon canker, disease resistance
- Inoculation methods for Populus tremuloides resistant to Hypoxylon canker
- Scale-dependence of desease impacts on quaking aspen (Populus tremuloides) mortality risk in the southwestern U.S.
- Growth of nitrogen-fertilized and thinned quaking aspen (Populus tremuloides Michx.).
XML: View XML