Skip to Main Content
U.S. Forest Service
Caring for the land and serving people

United States Department of Agriculture

Home > Search > Publication Information

  1. Share via EmailShare on FacebookShare on LinkedInShare on Twitter
    Dislike this pubLike this pub
    Author(s): S. A. Enebak; Michael E. Ostry; N. A. Anderson
    Date: 1999
    Source: Canadian Journal of Forest Research. 29(8): 1192-1196. (1999)
    Publication Series: Scientific Journal (JRNL)
    Station: North Central Research Station
    PDF: Download Publication  (782.27 KB)


    Canker expansion and the amount of callus tissue formed were measured monthly on 60 ramets from each of five trembling aspen (Populus tremuloides Michx.) clones that had been inoculated in wounds with Entoleuca mammata (= Hypoxylon marnmatum (Wahl.) Mill) over a 12-month period. At the clone level, the prevalence of nonlethal cankers within clones prior to the study had no correlation with canker expansion with three of the five clones. Greenhouse inoculation of ramets derived from the same five clones resulted in the same resistance rankings as main-stem inoculations in the field. One isolate, Hm-27, produced longer cankers, and less callus developed on all clones compared with trees inoculated with the less aggressive strain, Hm-24. Cankers developed only on clones that were inoculated during the months of April through July with April inoculations resulting in the largest cankers. These results indicate that there is a limited time frame when wounds on aspen are susceptible to infection by artificial inoculation with the pathogen as many wound inoculations neither produced cankers nor wound callus. Comparing inoculation methods, either main-stem inoculations in the field or greenhouse inoculations coupled with the natural canker prevalence could be used to include or exclude clones for use in an aspen breeding program.

    Publication Notes

    • Check the Northern Research Station web site to request a printed copy of this publication.
    • Our on-line publications are scanned and captured using Adobe Acrobat.
    • During the capture process some typographical errors may occur.
    • Please contact Sharon Hobrla, if you notice any errors which make this publication unusable.
    • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.


    Enebak, S. A.; Ostry, Michael E.; Anderson, N. A. 1999. Inoculation methods for Populus tremuloides resistant to Hypoxylon canker. Canadian Journal of Forest Research. 29(8): 1192-1196. (1999)


    Canker, trembling aspen, clones, greenhouse, aspen breeding program

    Related Search

    XML: View XML
Show More
Show Fewer
Jump to Top of Page