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Cloning and sequencing of a cellobiohydrolase gene from Trichoderma harzianum FP108Author(s): Patrick Guilfoile; Ron Burns; Zu-Yi Gu; Matt Amundson; Fu-Hsian Chang
Source: Journal of the Minnesota Academy of Science. 64(1): 18-22. (1999)
Publication Series: Scientific Journal (JRNL)
Station: North Central Research Station
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DescriptionA cbbl cellobiohydrolase gene was cloned and sequenced from the fungus Trichoderrna harzianum FP108. The cloning was performed by PCR amplification of T. harzianum genomic DNA, using PCR primers whose sequence was based on the cbbl gene from Tricboderma reesei. The 3' end of the gene was isolated by inverse PCR; attempts to clone regions upstream of the 5' end of the gene were unsuccessful. Sequence comparisons suggest that this gene is closely related to cbbl genes from other Trichoderma species. In particular, all catalytically important amino acids in the protein sequence deduced from the T. harzianum cbbl gene are conserved between species.
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CitationGuilfoile, Patrick; Burns, Ron; Gu, Zu-Yi; Amundson, Matt; Chang, Fu-Hsian 1999. Cloning and sequencing of a cellobiohydrolase gene from Trichoderma harzianum FP108. Journal of the Minnesota Academy of Science. 64(1): 18-22. (1999)
Keywordscloning, cellobiohydrolase, Trichoderma harzianum, DNA, genes
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