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    Author(s): C. Dana Nelson; Sedley Josserand; Craig S. Echt; Jeff Koppelman
    Date: 2007
    Source: In: Kabrick, John M.; Dey, Daniel C.; Gwaze, David, eds. Shortleaf pine restoration and ecology in the Ozarks: proceedings of a symposium; 2006 November 7-9; Springfield, MO. Gen. Tech. Rep. NRS-P-15. Newtown Square, PA: U.S. Department of Agriculture, Forest Service, Northern Research Station: 95-98.
    Publication Series: General Technical Report - Proceedings
    Station: Northern Research Station
    PDF: View PDF  (84.72 KB)

    Description

    Simple sequence repeats (SSR) are highly informative DNA-based markers widely used in population genetic and linkage mapping studies. We have been developing PCR primer pairs for amplifying SSR markers for loblolly pine (Pinus taeda L.) using loblolly pine DNA and EST sequence data as starting materials. Fifty primer pairs known to reliably amplify polymorphic markers in loblolly pine were screened for their use in shortleaf pine (P. echinata Mill.), longleaf pine (P. palustris Mill.), and slash pine (P. elliottii var. elliottii Engelm.). Thirty-four of these generated "high-quality" marker data for a small range-wide sample of shortleaf pines and 32 were polymorphic. Expected heterozygosities for the polymorphic markers averaged 0.71 and ranged from 0.40 to 0.88. A subset of the polymorphic markers should be very useful for determining identity or parentage of unknown trees while the whole set should provide excellent information on genetic diversity, gene flow, and population structure in shortleaf pine.

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    Citation

    Nelson, C. Dana; Josserand, Sedley; Echt, Craig S.; Koppelman, Jeff. 2007. Loblolly pine SSR markers for shortleaf pine genetics. In: Kabrick, John M.; Dey, Daniel C.; Gwaze, David, eds. Shortleaf pine restoration and ecology in the Ozarks: proceedings of a symposium; 2006 November 7-9; Springfield, MO. Gen. Tech. Rep. NRS-P-15. Newtown Square, PA: U.S. Department of Agriculture, Forest Service, Northern Research Station: 95-98.

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