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    Author(s): Narul Islam-Faridi; M. Abdul Majik; C. Dana Nelson
    Date: 2007
    Source: In: Kabrick, John M.; Dey, Daniel C.; Gwaze, David, eds. Shortleaf pine restoration and ecology in the Ozarks: proceedings of a symposium; 2006 November 7-9; Springfield, MO. Gen. Tech. Rep. NRS-P-15. Newtown Square, PA: U.S. Department of Agriculture, Forest Service, Northern Research Station: 99-101.
    Publication Series: General Technical Report - Proceedings
    Station: Northern Research Station
    PDF: View PDF  (141.45 KB)

    Description

    A reference karyotype (i.e., chromosome-specific description of a species' chromosomal complement) is a pre-requisite for advanced genetic and genomic studies. The Southern Institute of Forest Genetics has initiated a project to develop reference karyotypes for each of the major southern U.S. pine species, including shortleaf pine, using AT-rich chromosomal banding and fluorescent in situ hybridization (FISH). About half of the project has been completed to date, including the development of karyotypes for loblolly pine and slash pine, with the remaining experiments being directed towards shortleaf pine and longleaf pine. Preliminary FISH results for rDNA genes in shortleaf pine show that there are seven major, and as many as eight medium-to-minor centromeric 18S-28S rDNA sites. In addition, one major and one minor 5S rDNA sites were observed and most of the chromosomes showed AT-rich bands. A complete shortleaf pine karyotype is being developed for comparison with other pine and conifer species.

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    Citation

    Islam-Faridi, Narul; Majik, M. Abdul; Nelson, C. Dana. 2007. Chromosomal locations of the ribosomal dna genes in shortleaf pine. In: Kabrick, John M.; Dey, Daniel C.; Gwaze, David, eds. Shortleaf pine restoration and ecology in the Ozarks: proceedings of a symposium; 2006 November 7-9; Springfield, MO. Gen. Tech. Rep. NRS-P-15. Newtown Square, PA: U.S. Department of Agriculture, Forest Service, Northern Research Station: 99-101.

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