Skip to Main Content
U.S. Forest Service
Caring for the land and serving people

United States Department of Agriculture

Home > Search > Publication Information

 Due to a lapse in federal funding, this USDA website will not be actively updated. Once funding has been reestablished, online operations will continue.


  1. Share via EmailShare on FacebookShare on LinkedInShare on Twitter
    Dislike this pubLike this pub
    Author(s): Rakesh Minocha; Carolyn McQuattie; Wayne Fagerberg; Stephanie Long; Eun Woon Noh
    Date: 2001
    Source: Physiologia Plantarum. 113: 486-498.
    Publication Series: Scientific Journal (JRNL)
    Station: Northern Research Station
    PDF: View PDF  (3.67 MB)

    Description

    The effects of Al on red spruce (Picea rubens Sarg.) cell suspension cultures were examined using biochemical, stereo-logical and microscopic methods. Exposure to Al for 24-48 h resulted in a loss of cell viability, inhibition of growth and a significant decrease in mitochondrial activity. Soluble protein content increased in cells treated with Al. Using energy-dispersive X-ray microanalysis on sections of freeze-substituted cells that had no obvious disruption in cytoplasmic or cell wall structure, Al (always in the presence of P) was detected in dense regions in cell walls, cytoplasm, plastids and vacuoles after 48 h exposure to Al. Stereological quantification of spruce cell structure showed that, after 24 h of Al treatment, intact cells had increased vacuolar and total cell volume, but the nuclear volume did not change. In addition, Al treatment resulted in increased surface area of Golgi membranes and endoplasmic reticulum. The biochemical and ultrastructural alterations in red spruce cells, in combination with the presence of Al in cellular organelles of visually intact cells, suggest that Al movement occurred across the plasma membrane without major cellular disruption. Detailed short-term time course studies are needed to determine if intracellular Al in these cells results from its passage into cells through sub-microscopic lesions in the plasma membrane or it is taken up into the symplast through the intact membrane by an active, but slow, process.

    Publication Notes

    • Check the Northern Research Station web site to request a printed copy of this publication.
    • Our on-line publications are scanned and captured using Adobe Acrobat.
    • During the capture process some typographical errors may occur.
    • Please contact Sharon Hobrla, shobrla@fs.fed.us if you notice any errors which make this publication unusable.
    • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

    Citation

    Minocha, Rakesh; McQuattie, Carolyn; Fagerberg, Wayne; Long, Stephanie; Noh, Eun Woon. 2001. Effects of aluminum in red spruce (Picea rubens) cell cultures: Cell growth and viability, mitochondrial activity, ultrastructure and potential sites of intracellular aluminum accumulation. Physiologia Plantarum. 113: 486-498.

    Related Search


    XML: View XML
Show More
Show Fewer
Jump to Top of Page
https://www.fs.usda.gov/treesearch/pubs/13048