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High-performance liquid chromatographic method for the determination of dansyl-polyaminesAuthor(s): Subhash C. Minocha; Rakesh Minocha; Cheryl A. Robie
Source: Journal of Chromatography. 511: 177-183.
Publication Series: Scientific Journal (JRNL)
Station: Northern Research Station
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DescriptionThis paper describes a fast reliable, and a sensitive technique for the separation and quantification of dansylated polyamines by high-performance liquid chromatography. Using a small 33 x 4.6 mm I.D., 3 ?m particle size, C18 reversed-phase cartridge column and a linear gradient of acetonitrile-heptanesulfonate (10 mM, pH 3.4), at a flow-rate of 2.5 ml/min, the retention time for different polyamines was: N8-acetyl-spermidine, 1.79 min; N1-acetylspermidine, 1.82 min; putrescine, 2.26 min; cadaverine, 2.43 min; heptanediamine, 2.83 min; spermidine, 3.42 min; and spermine, 4.41 min. With an additional column regeneration time of 3-4 min, the complete cycle per sample took less than 8 min at room temperature. Using a fluorescence detector, the lower limit of detection was less than 1 pmol per 6 ?l injection volume. The fluorescence response was linear up to 200 pmol per 6 ?1 for each polyamine. The method is suitable for separation of polyamines from animal, plant and fungal sources.
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CitationMinocha, Subhash C.; Minocha, Rakesh; Robie, Cheryl A. 1990. High-performance liquid chromatographic method for the determination of dansyl-polyamines. Journal of Chromatography. 511: 177-183.
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