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Physiological responses of suspension cultures of Catharanthus roseus to aluminum: changes in polyamines and inorganic ionsAuthor(s): Xinhua Zhou; Rakesh Minocha; Subhash C. Minocha
Source: Journal of Plant Physiology. 145: 277-284.
Publication Series: Scientific Journal (JRNL)
Station: Northern Research Station
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DescriptionThe effects of aluminum (Al) treatment on polyamines were studied using suspension cultures of Madagascar periwinkle [Catharanthus roseus (L.) G. Don]. The addition of A1 (0.2, 0.5, 1.0 mM) to the suspension cultures caused a significant increase in putrescine within 24h only in freshly transferred cells. By contrast, Al treatment reduced putrescine content at all other times (days 2-7). Further analysis showed that the largest increase in putrescine occurred at 6h when Al was added at the time of transfer of cells to fresh medium. If Al treatment was delayed to as little as 3h after transfer to fresh medium, the cellular level of putrescine decreased. While spermidine remained unchanged, spermine was significantly higher in the presence of Al in all cases. The inhibitory effect of Al on DNA synthesis also depended upon the time of its addition. Cellular Ca, Mg, and Mn decreased in response to Al given at the time of transfer to fresh media while Fe increased, and Na, K and P remained unchanged. The results indicate a critical physiological change in the response of cells to Al within a short time (about 3h) after transfer to fresh medium.
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CitationZhou, Xinhua; Minocha, Rakesh; Minocha, Subhash C. 1995. Physiological responses of suspension cultures of Catharanthus roseus to aluminum: changes in polyamines and inorganic ions. Journal of Plant Physiology. 145: 277-284.
Keywordsarginine decarboxylase, Catharunthus roseus, DNA synthesis, inorganic ions, polyamines, putrescine, spermidine, spermine
- Regulation of DNA synthesis and cell division by polyamines in Catharanthus roseus suspension cultures
- Effects of aluminum on DNA synthesis, cellular polyamines, polyamine biosynthetic enzymes and inorganic ions in cell suspension cultures of a woody plant, Catharanthus roseus
- Effects of aluminum on organic acid metabolism and secretion by red spruce cell suspension cultures and the reversal of Al effects on growth and polyamine metabolism by exogenous organic acids
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