Skip to Main Content
U.S. Forest Service
Caring for the land and serving people

United States Department of Agriculture

Home > Search > Publication Information

  1. Share via EmailShare on FacebookShare on LinkedInShare on Twitter
    Dislike this pubLike this pub
    Author(s): Rakesh Minocha; Walter C. Shortle; Daniel J. Jr. Coughin; Subhash C. Minocha
    Date: 1996
    Source: Canadian Journal of Forest Research. 26: 550-559.
    Publication Series: Scientific Journal (JRNL)
    Station: Northern Research Station
    PDF: Download Publication  (771.93 KB)


    The influence of age of red spruce (Picea rubens Sarg.) cell suspensions on aluminum (Al) effects was studied by adding AICI3 (0.2, 0.5, and 1.0 mM) to the media on each day of a 7-day culture period and analyzing for changes in total cell mass, polyamines, arginine decarboxylase activity, and inorganic ions after 24 h of incubation. The effective concentrations of monomeric Al in the medium were found to be approximately 0.09, 0.23, and 0.48 mM, respectively. All levels of Al inhibited growth and increased cellular putrescine levels and arginine decarboxylase activity. Spermidine and spermine levels were either slightly elevated or not affected by Al. Cellular content of Ca, Mg, Mn, and K decreased, while Al and P increased in Al-treated cells. Aluminum effects, in general, were dose dependent. Since the response to 24 h incubation with Al was generally independent of culture age, 3-day-old cultures were selected to further study short- and long-term (4 h to 11 days) effects of Al. Cellular putrescine levels remained elevated in the Al-treated cells for the entire duration of the experiment. There was a sharp dose-dependent increase in spermine levels by the 7th day of incubation. After the initial decrease, Al treatments for longer than 2 to 4 days did not cause consistent effects on Ca, Mn, and Mg levels. Potassium decreased and P and Al increased in a dose-dependent manner over the 11-day culture period.

    Publication Notes

    • Check the Northern Research Station web site to request a printed copy of this publication.
    • Our on-line publications are scanned and captured using Adobe Acrobat.
    • During the capture process some typographical errors may occur.
    • Please contact Sharon Hobrla, if you notice any errors which make this publication unusable.
    • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.


    Minocha, Rakesh; Shortle, Walter C.; Coughin, Daniel J. Jr.; Minocha, Subhash C. 1996. Effects of aluminum on growth, polyamine metabolism, and inorganic ions in suspension cultures of red spruce (Picea rubens). Canadian Journal of Forest Research. 26: 550-559.

    Related Search

    XML: View XML
Show More
Show Fewer
Jump to Top of Page