Skip to Main Content
Polyamine levels during the development of zygotic and somatic embryos of Pinus radiataAuthor(s): Rakesh Minocha; Dale R. Smith; Cathie Reeves; Kevin D. Steele; Subhash C. Minocha
Source: Physiologia Plantarum. 105: 155-164.
Publication Series: Scientific Journal (JRNL)
Station: Northern Research Station
PDF: View PDF (745.12 KB)
DescriptionChanges in the cellular content of three polyamines (putrescine, spermidine and spermine) were compared at different stages of development in zygotic and somatic embryos of Pinus radiata D. Don. During embryo development, both the zygotic and the somatic embryos showed a steady increase in spermidine content, with either a small decrease or no significant change in putrescine. This led to a several-fold increase in spermidine/putrescine ratios during development of both types of embryos. Cell cultures of plant-forming and non-plantforming lines derived from the same clone and growing on proliferation (maintenance) medium differed significantly in their polyamine levels. Mature, cotyledonary stage somatic embryos capable of germination and formation of plants could be distinguished by their higher spermidine/putrescine ratios from abnormal cotyledonary stage somatic embryos which were incapable of forming plants.
- Check the Northern Research Station web site to request a printed copy of this publication.
- Our on-line publications are scanned and captured using Adobe Acrobat.
- During the capture process some typographical errors may occur.
- Please contact Sharon Hobrla, email@example.com if you notice any errors which make this publication unusable.
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationMinocha, Rakesh; Smith, Dale R.; Reeves, Cathie; Steele, Kevin D.; Minocha, Subhash C. 1999. Polyamine levels during the development of zygotic and somatic embryos of Pinus radiata. Physiologia Plantarum. 105: 155-164.
- Development of Screening Trials to Rank Pinus radiata Genotypes for Resistance to Defoliation by Monterey Pine Aphid (Essigella californica)
- Polyamines and their biosynthetic enzymes during somatic embryo development in red spruce (Picea rubens Sarg.)
- Contamination of Pinus radiata Seeds in California by Fusarium circinatum
XML: View XML