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    Author(s): Kenneth T. Belt; Christina Hohn; Aiah Gbakima; James A. Higgins
    Date: 2007
    Source: Journal of Water and Health. 5(3): 395-406.
    Publication Series: Scientific Journal (JRNL)
    Station: Northern Research Station
    PDF: View PDF  (349.05 KB)

    Description

    Bacteria present in water samples taken on a weekly basis, from June 2004 through June 2005, from three streams, were cultured on Coliscan® Easygel® agar plates. Colonies representative of a variety of colors and morphologies were subjected to amplification and sequencing of a 1000-1100 nt portion of the 16S rRNA gene. A total of 528 colonies were sequenced; these categorized into 26 genera and 78 species. Of 175 dark blue/purple colonies presumed to be E. coli, sequence analysis indicated that 45 (25%) were actually other genera.

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    Citation

    Belt, Kenneth T.; Hohn, Christina; Gbakima, Aiah; Higgins, James A. 2007. Identification of culturable stream water bacteria from urban, agricultural, and forested watersheds using 16S rRNA gene sequencing. Journal of Water and Health. 5(3): 395-406.

    Keywords

    16S rRNA, bacterial identification, freshwater bacteria, stream flora

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