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Identification of culturable stream water bacteria from urban, agricultural, and forested watersheds using 16S rRNA gene sequencingAuthor(s): Kenneth T. Belt; Christina Hohn; Aiah Gbakima; James A. Higgins
Source: Journal of Water and Health. 5(3): 395-406.
Publication Series: Scientific Journal (JRNL)
Station: Northern Research Station
PDF: Download Publication (349.05 KB)
DescriptionBacteria present in water samples taken on a weekly basis, from June 2004 through June 2005, from three streams, were cultured on Coliscan® Easygel® agar plates. Colonies representative of a variety of colors and morphologies were subjected to amplification and sequencing of a 1000-1100 nt portion of the 16S rRNA gene. A total of 528 colonies were sequenced; these categorized into 26 genera and 78 species. Of 175 dark blue/purple colonies presumed to be E. coli, sequence analysis indicated that 45 (25%) were actually other genera.
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CitationBelt, Kenneth T.; Hohn, Christina; Gbakima, Aiah; Higgins, James A. 2007. Identification of culturable stream water bacteria from urban, agricultural, and forested watersheds using 16S rRNA gene sequencing. Journal of Water and Health. 5(3): 395-406.
Keywords16S rRNA, bacterial identification, freshwater bacteria, stream flora
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