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  1. Isolation and characterization of juvenile hormone esterase from gypsy moth (Lymantria dispar)

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    Author(s): Algimantas P. Valaitis; Joan Jolliff
    Date: 1991
    Source: IN: Gottschalk, Kurt W.; Twery, Mark J.; Smith, Shirley I., eds. Proceedings, U.S. Department of Agriculture interagency gypsy moth research review 1990; East Windsor, CT. Gen. Tech. Rep. NE-146. Radnor, PA: U.S. Department of Agriculture, Forest Service, Northeastern Forest Experiment Station: 82.
    Publication Series: General Technical Report (GTR)
    Station: Northeastern Research Station
    PDF: View PDF  (69.72 KB)
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    Description

    Insect metamorphosis is under precise hormonal control. During the last larval stadium, the degradation of juvenile hormone by juvenile hormone esterase (JHE) is essential for the initiation of pupation. Therefore, we have targeted this system for disruption with a strategy to produce a recombinant gypsy moth virus which expresses JHE. In order to clone and insert the JHE gene into the virus, purification of the enzyme, amino acid sequence information, and polyclonal antibody were needed.

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    Citation

    Valaitis, Algimantas P.; Jolliff, Joan. 1991. Isolation and characterization of juvenile hormone esterase from gypsy moth (Lymantria dispar). IN: Gottschalk, Kurt W.; Twery, Mark J.; Smith, Shirley I., eds. Proceedings, U.S. Department of Agriculture interagency gypsy moth research review 1990; East Windsor, CT. Gen. Tech. Rep. NE-146. Radnor, PA: U.S. Department of Agriculture, Forest Service, Northeastern Forest Experiment Station: 82.

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