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Use of molecular probes to detect parasites and retrotransposons in gypsy moths

Author(s):

John H. Werren
Thomas O'Dell

Year:

1991

Publication type:

General Technical Report (GTR)

Primary Station(s):

Northern Research Station

Historical Station(s):

Northeastern Research Station

Source:

IN: Gottschalk, Kurt W.; Twery, Mark J.; Smith, Shirley I., eds. Proceedings, U.S. Department of Agriculture interagency gypsy moth research review 1990; East Windsor, CT. Gen. Tech. Rep. NE-146. Radnor, PA: U.S. Department of Agriculture, Forest Service, Northeastern Forest Experiment Station: 84.

Description

Retrotransposon screen: Gypsy moth families containing straggling and nonstraggling individuals were divided into categories of straggling, medium, and nonstraggling individuals, from which DNA was extracted. Four families were tested by southern hybridization and probing with ribosomal sequences designed to detect R1 and R2 retrotransposon insertions. Results showed no differences between stragglers and nonstragglers in the proportion of insertions in their ribosomal genes. The average proportion of insertions was 30%.

Citation

Werren, John H.; O''Dell, Thomas. 1991. Use of molecular probes to detect parasites and retrotransposons in gypsy moths. IN: Gottschalk, Kurt W.; Twery, Mark J.; Smith, Shirley I., eds. Proceedings, U.S. Department of Agriculture interagency gypsy moth research review 1990; East Windsor, CT. Gen. Tech. Rep. NE-146. Radnor, PA: U.S. Department of Agriculture, Forest Service, Northeastern Forest Experiment Station: 84.

Publication Notes

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https://www.fs.usda.gov/treesearch/pubs/14898