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  1. Use of molecular probes to detect parasites and retrotransposons in gypsy moths

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    Author(s): John H. Werren; Thomas O'Dell
    Date: 1991
    Source: IN: Gottschalk, Kurt W.; Twery, Mark J.; Smith, Shirley I., eds. Proceedings, U.S. Department of Agriculture interagency gypsy moth research review 1990; East Windsor, CT. Gen. Tech. Rep. NE-146. Radnor, PA: U.S. Department of Agriculture, Forest Service, Northeastern Forest Experiment Station: 84.
    Publication Series: General Technical Report (GTR)
    Station: Northeastern Research Station
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    Description

    Retrotransposon screen: Gypsy moth families containing straggling and nonstraggling individuals were divided into categories of straggling, medium, and nonstraggling individuals, from which DNA was extracted. Four families were tested by southern hybridization and probing with ribosomal sequences designed to detect R1 and R2 retrotransposon insertions. Results showed no differences between stragglers and nonstragglers in the proportion of insertions in their ribosomal genes. The average proportion of insertions was 30%.

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    Citation

    Werren, John H.; O''Dell, Thomas. 1991. Use of molecular probes to detect parasites and retrotransposons in gypsy moths. IN: Gottschalk, Kurt W.; Twery, Mark J.; Smith, Shirley I., eds. Proceedings, U.S. Department of Agriculture interagency gypsy moth research review 1990; East Windsor, CT. Gen. Tech. Rep. NE-146. Radnor, PA: U.S. Department of Agriculture, Forest Service, Northeastern Forest Experiment Station: 84.

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