Skip to Main Content
The application of 13C-labeled tetramethylammonium hydroxide (13C-TMAH) thermochemolysis to study fungal degradation of woodAuthor(s): T.R. Filley; P.G. Hatcher; W.C. Shortle
Source: Organic Geochemistry. 31: 181-198.
Publication Series: Scientific Journal (JRNL)
Station: Northern Research Station
PDF: Download Publication (286.05 KB)
DescriptionThis paper presents the results from an assessment of the application of a new molecular analytical procedure, 13C-TMAH thermochemolysis, to study the chemical modification of lignin by white-rot and brown-rot fungi. This technique differs from other molecular chemolysis procedures (e.g. TMAH thermochemolysis and CuO alkaline oxidation) as it enables one to determine the amount of hydroxylated aromatic components in degraded lignin residues through a selective lignin depolymerization and 13C-labeled methylation reaction. Major differences were observed in the chemical composition and yield of lignin monomers released from a limited sample set of field and laboratory inoculation brown-rot and white-rot degraded residues when analyzed by 13C-TMAH thermochemolysis. The brown-rot residues were characterized by high yields of 3,4-dihydroxy phenyl compounds, presumably due to fungal demethylation of methoxyl groups on guaiacyl lignin, and relatively low yields of aromatic acids that result from microbial side chain oxidation. The white-rot residues were characterized by low yields of demethylated lignin monomers but relatively high yields of monomers exhibiting side chain oxidation. If generally applicable, this distinct chemical functionality has important implications for the chemical reactivity and solubility of degraded wood residues and consequently the cycling of terrestrial carbon in the geosphere. The 13C-TMAH thermochemolysis procedure provides a rapid and sensitive tool for tracking microbial modifications of lignin in terrestrial environments including coastal sediments, forest soils and waters receiving terrestrial organic matter.
- Check the Northern Research Station web site to request a printed copy of this publication.
- Our on-line publications are scanned and captured using Adobe Acrobat.
- During the capture process some typographical errors may occur.
- Please contact Sharon Hobrla, firstname.lastname@example.org if you notice any errors which make this publication unusable.
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationFilley, T.R.; Hatcher, P.G.; Shortle, W.C. 2000. The application of 13C-labeled tetramethylammonium hydroxide (13C-TMAH) thermochemolysis to study fungal degradation of wood. Organic Geochemistry. 31: 181-198.
Keywords13C-Labeled tetramethylammonium hydroxide, demethylation, phenols, basidiomycetes, lignin, white-rot fungal degradation, brown-rot fungal degradation
- Evidence for cleavage of lignin by a brown rot basidiomycete
- Light-induced yellowing of selectively 13C-enriched dehydrogenation polymers (DHPs). Part 2, NMR assignments and photoyellowing of aromatic ring 1-, 3-, 4-, and 5-13C DHPs
- Role of fungal peroxidases in biological ligninolysis
XML: View XML