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  1. Relative quantification of membrane-associated calcium in red spruce mesophyll cells

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    Author(s): Catherine H. Borer; Paul Schaberg; Jonathan R. Cumming
    Date: 1997
    Source: Trees. 12: 21-26.
    Publication Series: Scientific Journal (JRNL)
    Station: Northern Research Station
    PDF: View PDF  (135.25 KB)

    Description

    We describe a method for localizing and comparing relative amounts of plasma membrane-associated calcium ions (mCa) in complex tissues and verify the procedure for mesophyll cells of red spruce (Picea rubens Sarg.) needles. This technique incorporates epifluorescence microscopy using the fluorescent probe chlorotetracycline (CTC) with computer image processing and analysis. Using an appropriate standardization for image brightness, the procedure allows relative quantitative comparison of CTC-fluorescence in the plasma membrane-cell wall region that corresponds to relative amounts of mCa. The technique effectively discerned mCa differences among red spruce needle sections exposed to treatments designed to alter mCa levels in vitro.

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    Citation

    Borer, Catherine H.; Schaberg, Paul; Cumming, Jonathan R. 1997. Relative quantification of membrane-associated calcium in red spruce mesophyll cells. Trees. 12: 21-26.

    Keywords

    red spruce, mesophyll cell, membrane-associated calcium (mCa), chlorotetracycline (CTC), fluorescence microscopy

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