Skip to Main Content
U.S. Forest Service
Caring for the land and serving people

United States Department of Agriculture

Home > Search > Publication Information

  1. Share via EmailShare on FacebookShare on LinkedInShare on Twitter
    Dislike this pubLike this pub
    Author(s): Erin Victory; Rodney L. RobichaudKeith Woeste
    Date: 2003
    Source: In: Van Sambeek, J. W.; Dawson, Jeffery O.; Ponder Jr., Felix; Loewenstein, Edward F.; Fralish, James S., eds. Proceedings of the 13th Central Hardwood Forest Conference; Gen. Tech. Rep. NC-234. St. Paul, MN: U.S. Department of Agriculture, Forest Service, North Central Research Station: 466
    Publication Series: General Technical Report (GTR)
    Station: North Central Research Station
    PDF: View PDF  (24.57 KB)

    Description

    Chloroplasts are a valuable source of genetic information because their sequence is highly conserved, they undergo little or no recombination, and they are uniparentally inherited. Chloroplast polymorphisms are powerful genetic tools for identifying matrilineal family groups, studying gene flow from seed versus pollen movement, reconstructing phylogeographic colonization, and for elucidating reproductive patterns in natural stands. Here, we sequenced six samples representing the range of black walnut (Juglans nigra L.) using six previously published polymorphic chloroplast markers. These markers were from the atpF intron, 3' to rps2, ORF 77-ORF 82 intergenic, ORF 74b-psbB intergenic, and rp12-rps19 intergenic regions in the chloroplast genome. Data from Weising and Gardner (1999) show short mononucleotide repeats of A, T, and G, both individually and in tandem depending on locus. Preliminary data suggest that in black walnut, polymorphisms in these regions are based on series of A and T repeats, and for some loci indicate considerable differences from all the species previously analyzed. New primers specific for black walnut will be designed based on these polymorphisms. These new primers will be used in a study of genetic diversity across the Central Hardwood region.

    Publication Notes

    • Check the Northern Research Station web site to request a printed copy of this publication.
    • Our on-line publications are scanned and captured using Adobe Acrobat.
    • During the capture process some typographical errors may occur.
    • Please contact Sharon Hobrla, shobrla@fs.fed.us if you notice any errors which make this publication unusable.
    • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

    Citation

    Victory, Erin; Robichaud, Rodney L.; Woeste, Keith. 2003. Designing specific chloroplast markers for black walnut from a set of universal primers. In: Van Sambeek, J. W.; Dawson, Jeffery O.; Ponder Jr., Felix; Loewenstein, Edward F.; Fralish, James S., eds. Proceedings of the 13th Central Hardwood Forest Conference; Gen. Tech. Rep. NC-234. St. Paul, MN: U.S. Department of Agriculture, Forest Service, North Central Research Station: 466

    Related Search


    XML: View XML
Show More
Show Fewer
Jump to Top of Page
https://www.fs.usda.gov/treesearch/pubs/15824