Skip to Main Content
U.S. Forest Service
Caring for the land and serving people

United States Department of Agriculture

Home > Search > Publication Information

  1. Share via EmailShare on FacebookShare on LinkedInShare on Twitter
    Dislike this pubLike this pub
    Author(s): Xiaomei Liu; Paula M. Pijut
    Date: 2008
    Source: Plant Cell, Tissue and Organ Culture. 94: 113-123.
    Publication Series: Scientific Journal (JRNL)
    Station: Northern Research Station
    PDF: Download Publication  (338.35 KB)


    A regeneration system was developed for Prunus serotina from a juvenile (F) and two mature genotypes (#3 and #4). Adventitious shoots regenerated from leaves of in vitro cultures on woody plant medium with thidiazuron (TDZ) and naphthaleneacetic acid (NAA). The best regeneration for genotype F (91.4%) was observed on medium with 9.08 µM TDZ and 1.07 µM NAA. The highest mean number of shoots (8.2) was obtained on medium containing 9.08 µM TDZ and 0.54 µM NAA. Genotype #3 had the highest regeneration (41.7%) with a mean number of shoots (4.8) on 9.08 µM TDZ and 1.07 µM NAA, whereas genotype #4 had a 38.8% regeneration with a mean of 3.3 shoots. Genotype #4 had the highest mean number of shoots (4.8) on 4.54 µM TDZ and 1.07 µM NAA. Silver thiosulphate at 60 or 80 µM increased the percent regeneration of the mature genotypes #3 (75%) and #4 (58%). Adventious shoots were rooted (70-76%) and rooted plantlets survived after acclimatization to the greenhouse. The effect of kanamycin concentration on adventitious shoot regeneration was also evaluated.

    Publication Notes

    • Check the Northern Research Station web site to request a printed copy of this publication.
    • Our on-line publications are scanned and captured using Adobe Acrobat.
    • During the capture process some typographical errors may occur.
    • Please contact Sharon Hobrla, if you notice any errors which make this publication unusable.
    • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.


    Liu, Xiaomei; Pijut, Paula M. 2008. Plant regeneration from in vitro leaves of mature black cherry (Prunus serotina). Plant Cell, Tissue and Organ Culture. 94: 113-123.


    adventitious shoots, organogenesis, rooting, silver thiosulphate

    Related Search

    XML: View XML
Show More
Show Fewer
Jump to Top of Page