Skip to Main Content
U.S. Forest Service
Caring for the land and serving people

United States Department of Agriculture

Home > Search > Publication Information

  1. Share via EmailShare on FacebookShare on LinkedInShare on Twitter
    Dislike this pubLike this pub
    Author(s): Rakesh Minocha; P. Thangavel; Om Parkash Dhankher; Stephanie Long
    Date: 2008
    Source: Journal of Chromatography A. 1207: 72-83.
    Publication Series: Scientific Journal (JRNL)
    Station: Northern Research Station
    PDF: View PDF  (1.35 MB)

    Description

    The HPLC method presented here for the quantification of metal-binding thiols is considerably shorter than most previously published methods. It is a sensitive and highly reproducible method that separates monobromobimane tagged monothiols (cysteine, glutathione, γ-glutamylcysteine) along with polythiols (PC2, PC3, PC4 and PC5 within 23 min from a wide variety of samples. Total run time of the method is 35 min. Detection limits for thiols is 33 fmol for µL injection. This method will be applicable to study the metal detoxification mechanisms for a wide variety of cell cultures and tissues of plants and trees including algae, Arabidopsis, crambe, rice, and red spruce.

    Publication Notes

    • Check the Northern Research Station web site to request a printed copy of this publication.
    • Our on-line publications are scanned and captured using Adobe Acrobat.
    • During the capture process some typographical errors may occur.
    • Please contact Sharon Hobrla, shobrla@fs.fed.us if you notice any errors which make this publication unusable.
    • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

    Citation

    Minocha, Rakesh; Thangavel, P.; Dhankher, Om Parkash; Long, Stephanie. 2008. Separation and quantification of monothiols and phytochelatins from a wide variety of cell cultures and tissues of trees and other plants using high performance liquid chromatography. Journal of Chromatography A. 1207: 72-83.

    Keywords

    algae, Arabidopsis, Crambe, forest trees, HPLC, mBBr, phytochelatins, sugar maple, red spruce, rice

    Related Search


    XML: View XML
Show More
Show Fewer
Jump to Top of Page
https://www.fs.usda.gov/treesearch/pubs/19731