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    Author(s): G. Vengadesan; Paula M. Pijut
    Date: 2009
    Source: In Vitro Cellular and Developmental Biology-Plant. 45: 474-482.
    Publication Series: Scientific Journal (JRNL)
    Station: Northern Research Station
    PDF: View PDF  (261.32 KB)

    Description

    In vitro propagation of northern red oak (Quercus rubra) shoots was successful from cotyledonary node explants excised from 8-wk-old in vitro grown seedlings. Initially, four shoots per explant were obtained on Murashige and Skoog (MS) medium supplemented with 4.4 µM 6-benzylaminopurine (BA), 0.45 µM thidiazuron (TDZ), and 500 mg l−1 casein hydrolysate (CH) with a regeneration frequency of 64.7% after 3 wk. Subculturing explants (after harvesting shoots) to fresh treatment medium significantly increased shoot bud regeneration (16.6 buds per explant), but the buds failed to develop into shoots.

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    Citation

    Vengadesan, G.; Pijut, Paula M. 2009. In vitro propagation of northern red oak (Quercus rubra L.). In Vitro Cellular and Developmental Biology-Plant. 45: 474-482.

    Keywords

    cotyledonary node culture, micropropagation, plant regeneration, rooting

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