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    Author(s): Ningxia Du; Paula M. Pijut
    Date: 2009
    Source: Plant Cell Reports. 28: 915-923.
    Publication Series: Scientific Journal (JRNL)
    Station: Northern Research Station
    PDF: View PDF  (313.82 KB)

    Description

    A genetic transformation protocol for green ash (Fraxinus pennsylvanica) hypocotyl explants was developed. Green ash hypocotyls were transformed using Agrobacterium tumefaciens strain EHA105 harboring binary vector pq35GR containing the neomycin phosphotransferase (nptII) and β-glucuronidase (GUS) fusion gene, and an enhanced green fluorescent protein gene. Pre-cultured hypocotyl explants were transformed in the presence of 100 µM acetosyringone using 90 s sonication plus 10 min vacuum-infiltration.

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    Citation

    Du, Ningxia; Pijut, Paula M. 2009. Agrobacterium-mediated transformation of Fraxinus pennsylvanica hypocotyls and plant regeneration. Plant Cell Reports. 28: 915-923.

    Keywords

    genetic engineering, green ash, sonication, transgenic, vacuum-infiltration

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