Skip to Main Content
U.S. Forest Service
Caring for the land and serving people

United States Department of Agriculture

Home > Search > Publication Information

  1. Share via EmailShare on FacebookShare on LinkedInShare on Twitter
    Dislike this pubLike this pub
    Author(s): Annette M. Kretzer; Susie Dunham; Randy Molina; Joseph W. Spatafora
    Date: 2003
    Source: New Phytologist. 161: 313-320
    Publication Series: Scientific Journal (JRNL)
    PDF: Download Publication  (642 KB)


    We have developed microsatellite markers for two sister species of Rhizopogon, R. vesiculosus and R. vinicolor (Boletales, Basidiomycota), and used selected markers to investigate genet size and distribution from ectomycorrhizal samples. Both species form ectomycorrhizas with tuberculate morphology on Douglas-fir (Pseudotsuga menziesii).

    Tuberculate ectomycorrhizas were sampled and mapped in two 10 x 10 m core plots located at Mary's Peak in the Oregon Coast Range and at Mill Creek in the Oregon Cascade Mountains, USA; additional samples were obtained from a larger area surrounding the Mary's Peak core plot.

    Gene diversities at the newly described microsatellite loci ranged from 0.00 to 0.68 in R. vesiculosus, and from 0.00 to 0.43 in R. vinicolor. Both taxa appeared to be in Hardy-Weinberg and linkage equilibrium. The largest distance observed between tuberculate ectomycorrhizas of the same genet was 13.4 m for R. vesiculosus, but only 2 m for R. vinicolor.

    This is to our knowledge the first study to differentiate fungal genets from ectomycorrhizas with great confidence using multiple codominant markers.

    Publication Notes

    • Visit PNW's Publication Request Page to request a hard copy of this publication.
    • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.


    Kretzer, Annette M.; Dunham, Susie; Molina, Randy; Spatafora, Joseph W. 2003. Microsatellite markers reveal the below ground distribution of genets in two species of Rhizopogon forming tuberculate ectomycorrhizas on Douglas-fir. New Phytologist. 161: 313-320


    Rhizopogon, tuberculate, ectomycorrhizas, fungal genets, microsatellite markers

    Related Search

    XML: View XML
Show More
Show Fewer
Jump to Top of Page