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    Author(s): Mauro E. Martignoni; Paul J. Iwai
    Date: 1981
    Source: Res. Pap. PNW-RP-285. Portland, OR: U.S. Department of Agriculture, Forest Service. 20 p.
    Publication Series: Research Paper (RP)
    Station: Pacific Northwest Research Station
    PDF: View PDF  (1.64 MB)

    Description

    The relative virulence of entomopathogenic viruses and the potency of virus preparations for control of destructive insects can be estimated reliably only by means of biological assay in the target species. A simple, yet sensitive peroral bioassay procedure is described for preparations of nucleopolyhedrosis viruses pathogenic for the western spruce budworm, Choristoneura occidentalis Freeman. This procedure has been used to compare the activity of Baculovirus isolates from several conifer-feeding species of Choristoneura.

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    Citation

    Martignoni, Mauro E.; Iwai, Paul J. 1981. Peroral bioassay of nucleopolyhedrosis viruses in larvae of western spruce budworm. Res. Pap. PNW-RP-285. Portland, OR: U.S. Department of Agriculture, Forest Service, Pacific Northwest Forest and Range Experiment Station. 20 p.

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    Keywords

    Bioassay, Baculovirus, nucleopolyhedrosis virus, virus (insect), diseases (insect), quality control (virus preparations), biological control (forest pests), western spruce budworm, Choristoneura occidentalis

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