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Xylitol production by a Pichia stipitis D-xylulokinase mutantAuthor(s): Yong-Su Jin; Jose Cruz; Thomas W. Jeffries
Source: Applied microbiology and biotechnology. Vol. 68, no. 1 (2005): pages 42-45.
Publication Series: Miscellaneous Publication
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DescriptionXylitol production by Pichia stipitis FPL-YS30, a xyl3-Ä1 mutant that metabolizes xylose using an alternative metabolic pathway, was investigated under aerobic and oxygen-limited culture conditions. Under both culture conditions, FPL-YS30 (xyl3-Ä1) produced a negligible amount of ethanol and converted xylose mainly into xylitol with comparable yields (0.30 and 0.27 g xylitol/g xylose). However, xylose consumption increased five-fold under aerobic compared to oxygen-limited conditions. This suggests that the efficiency of the alternative route of xylose assimilation is affected by respiration. As a result, the FPL-YS30 strain produced 26 g/l of xylitol, and exhibited a higher volumetric productivity (0.22 g xylitol l−1 h−1) under aerobic conditions.
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CitationJin, Yong-Su; Cruz, Jose; Jeffries, Thomas W. 2005. Xylitol production by a Pichia stipitis D-xylulokinase mutant. Applied microbiology and biotechnology. Vol. 68, no. 1 (2005): pages 42-45.
KeywordsFungi, cultures, culture media, genetic engineering, biotechnology, alcohol, xylitol, Pichia stipitis, xylose, D-xylulokinase
- Fermentation kinetics for xylitol production by a Pichia stipitis D-xylulokinase mutant previously grown in spent sulfite liquor
- Saccharomyces cerevisiae engineered for xylose metabolism exhibits a respiratory response
- Stoichiometric network constraints on xylose metabolism by recombinant Saccharomyces cerevisiae
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