Skip to Main Content
Analysis of D-glucose metabolism of wood decay fungi using 13C-NMR and 13C-labeled substratesAuthor(s): Theodorus H. de Koker; Michael D. Mozuch; Philip J. Kersten
Source: Thirty-fourth annual meeting of the International Research Group on Wood Preservation : 2003 May 18-23, Brisbane, Queensland, Australia. Stockholm, Sweden : IRG Secretariat, 2003: 9 pages.
Publication Series: Miscellaneous Publication
PDF: Download Publication (129 KB)
DescriptionD-Glucose metabolism is thought to be important during wood decay by fungi, not only for anabolic and catabolic purposes of central metabolism, but also as a potential source of peroxide required by extracellular peroxidases. There has been some confusion in the literature as to whether this peroxide-generating activity is of the glucose 1-oxidase or pyranose 2-oxidase (glucose 2-oxidase) type with various fungi or even within the same fungal species. Definitive classification requires accurate identification of the enzymatic products D-glucono- 1,5-lactone and D- arabino-2-hexosulose (glucosone) with glucose 1-oxidase and pyranose 2- oxidase, respectively. We used 13C-NMR to distinguish these reactions starting with 13C-labeled glucose. The use of labeled substrates simplifies analysis and greatly increases detection sensitivity without requiring the isolation or derivatization of metabolites. We synthesized 13C-1-glucosone to study subsequent metabolism with crude enzyme preparations. Preliminary results with Phanerochaete chrysosporium are presented.
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
Citationde Koker, Theodorus H.; Mozuch, Michael D.; Kersten, Philip J. 2003. Analysis of D-glucose metabolism of wood decay fungi using 13C-NMR and 13C-labeled substrates. Thirty-fourth annual meeting of the International Research Group on Wood Preservation : 2003 May 18-23, Brisbane, Queensland, Australia. Stockholm, Sweden : IRG Secretariat, 2003: 9 pages.
KeywordsPhanerochaete chrysosporium, glucose 1-oxidase, pyranose 2-oxidase, D-glucono-1, 5-lactone, glucosone, pyranosone dehydratase, wood-decaying fungi, peroxidase, microbial metabolism, microbial metabolites, carbon, fungal physiology, nuclear magnetic resonance, enzymes, Phanerochaete chrysosporium
- Isolation and purification of pyranose 2-oxidase from Phanerochaete chrysosporium and characterization of gene structure and regulation
- Gene cloning and heterologous expression of pyranose 2-oxidase from the brown-rot fungus, Gloeophyllum trabeum
- Fungal carbon sources in a pine forest: evidence from a 13C-labeled global change experiment
XML: View XML