Skip to Main Content
U.S. Forest Service
Caring for the land and serving people

United States Department of Agriculture

Home > Search > Publication Information

  1. Share via EmailShare on FacebookShare on LinkedInShare on Twitter
    Dislike this pubLike this pub
    Author(s): Hongwen Huang; Fenny Dane; Thomas L. Kubisiak
    Date: 1998
    Source: American Journal of Botany 85(7): 1013-1021. 1998.
    Publication Series: Miscellaneous Publication
    PDF: View PDF  (226 KB)


    Genetic variation among 12 populations of the American chestnut (Custanea dentata) was investigated. Population genetic parameters estimated from allozyme variation suggest that C. dentata at both the population and species level has narrow genetic diversity as compared to other species in the genus. Average expected heterozygosity was relatively low for the population collected in the Black Rock Mountain State Park, Georgia (He = 0.096 ± 0.035), and high for the population in east central Alabama (He = 0.196 ± 0.048). Partitioning of the genetic diversity based on 18 isozyme loci showed that -10% of the allozyme diversity resided among populations. Cluster analysis using unweighted pair-group method using arithmetric averages of Rogers' genetic distance and principal components analysis based on allele frequencies of both isozyme and RAPD loci revealed four groups: the southernmost population, south-central Appalachian populations, north- central Appalachian populations, and northern Appalachian populations. Based on results presented in this study, a conservation strategy and several recommendations related to the backcross breeding aimed at restoring C. dentata are discussed.

    Publication Notes

    • You may send email to to request a hard copy of this publication.
    • (Please specify exactly which publication you are requesting and your mailing address.)
    • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.


    Huang, Hongwen; Dane, Fenny; Kubisiak, Thomas L. 1998. Allozyme and RAPD Analysis of the Genetic Diversity and Geographic Variation in Wild Populations of the American Chestnut (Fagaceae). American Journal of Botany 85(7): 1013-1021. 1998.


    allozyme variation, Castanea dentata, conservation, Fagaceae, geographic variation, genetic diversity

    Related Search

    XML: View XML
Show More
Show Fewer
Jump to Top of Page