Skip to Main Content
DNA from bird-dispersed seed and wind-disseminated pollen provides insights into postglacial colonization and population genetic structure of whitebark pine (Pinus albicaulis)Author(s): Bryce A. Richardson; Steven J. Runsfeld; Ned B. Klopfenstein
Source: Molecular Ecology. 11(2): 215-227.
Publication Series: Scientific Journal (JRNL)
Station: Rocky Mountain Research Station
PDF: View PDF (1.14 MB)
DescriptionUniparentally inherited mitochondrial (mt)DNA and chloroplast (cp)DNA microsatellites (cpSSRs) were used to examine population genetic structure and biogeographic patterns of bird-dispersed seed and wind-disseminated pollen of whitebark pine (Pinus albicaulis Engelm.). Sampling was conducted from 41 populations throughout the range of the species. Analyses provide evidence for an ancestral haplotype and two derived mtDNA haplotypes with distinct regional distributions. An abrupt contact zone between mtDNA haplotypes in the Cascade Range suggests postglacial biogeographic movements. Among three cpSSR loci, 42 haplotypes were detected within 28 cpSSR sample populations that were aggregated into six regions. Analysis of molecular variance (AMOVA) was used to determine the hierarchical genetic structure of cpSSRs. AMOVA and population pairwise comparisons (FST) of cpSSR, and geographical distribution of mtDNA haplotypes provide insights into historical changes in biogeography. The genetic data suggest that whitebark pine has been intimately tied to climatic change and associated glaciation, which has led to range movements facilitated by seed dispersal by Clark's nutcracker (Nucifraga columbiana Wilson). The two hypotheses proposed to explain the genetic structure are: (i) a northward expansion into Canada and the northern Cascades in the early Holocene; and (ii) historical gene flow between Idaho and the Oregon Cascades when more continuous habitat existed in Central Oregon during the late Pleistocene. Genetic structure and insights gained from historical seed movements provide a basis on which to develop recovery plans for a species that is at risk from multiple threats.
- You may send email to firstname.lastname@example.org to request a hard copy of this publication.
- (Please specify exactly which publication you are requesting and your mailing address.)
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationRichardson, Bryce A.; Runsfeld, Steven J.; Klopfenstein, Ned B. 2002. DNA from bird-dispersed seed and wind-disseminated pollen provides insights into postglacial colonization and population genetic structure of whitebark pine (Pinus albicaulis). Molecular Ecology. 11(2): 215-227.
Keywordsbiogeography, chloroplast microsatellites, gene flow, mitochondrial DNA, Pinus albicaulis, postglacial colonization
- The influence of white pine blister rust on seed dispersal in whitebark pine
- Assessing Clark's nutcracker seed-caching flights using maternally inherited mitochondrial DNA of whitebark pine
- Determining Clark's nutcracker use of whitebark pine communities in regard to stand health in Waterton-Glacier International Peace Park
XML: View XML