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Effects of coumarate 3-hydroxylase down-regulation on lignin structureAuthor(s): John Ralph; Takuya Akiyama; Hoon Kim; Fachuang Lu; Paul F. Schatz; Jane M. Marita; Sally A. Ralph; M.S. Srinivasa Reddy; Fang Chen; Richard A. Dixon
Source: Journal of biological chemistry. Vol. 281, no. 13 (Mar. 31, 2006): pages 8843-8853
Publication Series: Miscellaneous Publication
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DescriptionDown-regulation of the gene encoding 4-coumarate 3-hydroxylase (C3H) in alfalfa massively but predictably increased the proportion of p-hydroxyphenyl (P) units relative to thenormally dominant guaiacyl (G) and syringyl (S) units Stem levels of up to ~65% P (from wild-type levels of ~1%) resulting from down-regulation of C3H were measured by traditional degradative analyses as well as two-dimensional 13C-1H correlative NMR methods. Such levels put these transgenics well beyond the P:G:S compositional bounds of normal plants; p-hydroxyphenyl levels are reported to reach a maximum of 30% in gymnosperm severe compression wood zones but are limited to a few percent in dicots. NMR also revealed structural differences in the interunit linkage distribution that characterizes a lignin polymer. Lower levels of key b-aryl ether units were relatively augmented by higher levels of phenylcoumarans and resinols. The C3H- eficient alfalfa lignins were devoid of b-1 coupling products, highlighting the significant differences in the reaction course for p- coumaryl alcohol versus the two normally dominant monolignols, coniferyl and sinapyl alcohols. A larger range of dibenzodioxocin structures was evident in conjunction with an approximate doubling of their proportion. The nature of each of the structural units was revealed by long range 13C-H correlation experiments. For example, although 8-ethers resulted from the coupling of all three monolignols with the growing polymer, phenylcoumarans were formed almost solely from coupling reactions involving p-coumaryl alcohol; they resulted from both coniferyl and sinapyl alcohol in the wild-type plants. Such structural differences form a basis for explaining differences in digestibility and pulping performance of C3H-deficient plants.
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CitationRalph, John; Akiyama, Takuya; Kim, Hoon; Lu, Fachuang; Schatz, Paul F.; Marita, Jane M.; Ralph, Sally A.; Reddy, M.S. Srinivasa; Chen, Fang; Dixon, Richard A. 2006. Effects of coumarate 3-hydroxylase down-regulation on lignin structure. Journal of biological chemistry. Vol. 281, no. 13 (Mar. 31, 2006): pages 8843-8853
KeywordsDown regulation, lignin structure, coumarate, hydroxylase
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