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    Author(s): M. A. Alexander; T. W. Chapman; T. W. Jeffries
    Date: 1988
    Source: Proceedings of the 9th symposium on biotechnology for fuels and chemicals. Clifton, N.J. : Humana Press, 1988. Applied biochemistry and biotechnology ; v. 17: pages 221-229.
    Publication Series: Miscellaneous Publication
    PDF: View PDF  (169 KB)

    Description

    Recent work has identified ethanol toxicity as a major factor preventing continuous production of ethanol at the concentrations obtainable in batch culture. In this paper we investigate the use of a continuous two-stage bioreactor design to circumvent toxic effects of ethanol. Biomass is produced via continuous culture in the first stage reactor in which ethanol concentrations are either absent or maintained at low levels. The freshly grown cells are fed into the second bioreactor in which high ethanol concentrations are produced. The steady influx of fresh cells and continuous removal of spent cells helps minimize the loss of fermentative activity that results from anaerobiosis and exposure to high ethanol concentrations. A final ethanol concentration of 37 g L-1 and overall yield of .32 g g-1 were obtained with the two-stage reactor as compared to corresponding values of 38 g L-1 and .32 g g-1 obtained in batch. The volumetric rate in the two-state process was .96 g L-1 as compared to .46 g L-1h-1 in batch.

    Publication Notes

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    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

    Citation

    Alexander, M. A.; Chapman, T. W.; Jeffries, T. W. 1988. Continuous xylose fermentation by Candida shehatae in a two-stage reactor. Proceedings of the 9th symposium on biotechnology for fuels and chemicals. Clifton, N.J. : Humana Press, 1988. Applied biochemistry and biotechnology ; v. 17: pages 221-229.

    Keywords

    Xylose fermentation, continuous fermentation, ethanol toxicity, Candida shehatae, anaerobic

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