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Development of a rapid and simple Agrobacterium tumefaciens mediated transformation system for the fungal pathogen Heterobasidion annosumAuthor(s): Nicklas Samils; Malin Elfstrand; Daniel L. Lindner Czederpiltz; Jan Fahleson; Ake Olson; Christina Dixelius; Jan Stenlid
Source: FEMS microbiology letters. Vol. 255 (2006): pages 82-88.
Publication Series: Miscellaneous Publication
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DescriptionHeterobasidion annosum causes root and butt-rot in trees and is the most serious forest pathogen in the northern hemisphere. We developed a rapid and simple Agrobacterium-mediated method of gene delivery into H. annosum to be used in functional studies of candidate genes and for visualization of mycelial interactions. Heterobasidion annosum TC 32-1 was cocultivated at pH 5.6 and 20 1C in Hagems medium with Agrobacterium tumefaciens C58 carrying plasmids with hygromycin B resistance as the selectable marker and green fluorescent protein as a visual marker. We obtained 18 mitotically stable transformed isolates showing green fluorescence protein activity.
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CitationSamils, Nicklas; Elfstrand, Malin; Czederpiltz, Daniel L. Lindner; Fahleson, Jan; Olson, Ake; Dixelius, Christina; Stenlid, Jan. 2006. Development of a rapid and simple Agrobacterium tumefaciens mediated transformation system for the fungal pathogen Heterobasidion annosum. FEMS microbiology letters. Vol. 255 (2006): pages 82-88.
KeywordsAgrobacterium tumefaciens, Heterobasidion annosum, green fluorescent protein (GFP), transformation, genetic markers, mycelium, phytopathogenic fungi, biological control, butt rots, pathogenic fungi, root rots, Basidiomycetes, genetic transformation, resistance to decay, decay fungi
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