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Rapid analysis of abietanes in conifersAuthor(s): P.J. Kersten; B.J. Kopper; K.F. Raffa; B.L. Illman
Source: Journal of chemical ecology. Vol. 32 (2006): pages 2679-2685.
Publication Series: Miscellaneous Publication
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DescriptionDiterpene resin acids are major constituents of conifer oleoresin and play important roles in tree defense against insects and microbial pathogens. The tricyclic C-20 carboxylic acids are generally classified into two groups, the abietanes and the pimaranes. The abietanes have conjugated double bonds and exhibit characteristic UV spectra. Here, we report the analysis of abietanes by reversed-phase high-performance liquid chromatography using multiwavelength detection to optimize quantification of underivatized abietic, neoabietic, palustric, levopimaric, and dehydroabietic acids. The utility of the method is demonstrated with methanol extracts of white spruce (Picea glauca) phloem, and representative concentrations are reported.
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CitationKersten, P.J.; Kopper, B.J.; Raffa, K.F.; Illman, B.L. 2006. Rapid analysis of abietanes in conifers. Journal of chemical ecology. Vol. 32 (2006): pages 2679-2685.
KeywordsAbietic, neoabietic, palustric, levopimaric, dehydroabietic, white spruce, diterpenes, HPLC, abietic acid, high performance liquid chromatography, oleoresins, carboxylic acids, dehydroabietic acid, neoabietic acid, palustric acid, pimaranes, abietanes, resin acids, levopimaric acid
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