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    Author(s): P.J. Kersten; B.J. Kopper; K.F. Raffa; B.L. Illman
    Date: 2006
    Source: Journal of chemical ecology. Vol. 32 (2006): pages 2679-2685.
    Publication Series: Miscellaneous Publication
    PDF: View PDF  (166 KB)

    Description

    Diterpene resin acids are major constituents of conifer oleoresin and play important roles in tree defense against insects and microbial pathogens. The tricyclic C-20 carboxylic acids are generally classified into two groups, the abietanes and the pimaranes. The abietanes have conjugated double bonds and exhibit characteristic UV spectra. Here, we report the analysis of abietanes by reversed-phase high-performance liquid chromatography using multiwavelength detection to optimize quantification of underivatized abietic, neoabietic, palustric, levopimaric, and dehydroabietic acids. The utility of the method is demonstrated with methanol extracts of white spruce (Picea glauca) phloem, and representative concentrations are reported.

    Publication Notes

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    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

    Citation

    Kersten, P.J.; Kopper, B.J.; Raffa, K.F.; Illman, B.L. 2006. Rapid analysis of abietanes in conifers. Journal of chemical ecology. Vol. 32 (2006): pages 2679-2685.

    Keywords

    Abietic, neoabietic, palustric, levopimaric, dehydroabietic, white spruce, diterpenes, HPLC, abietic acid, high performance liquid chromatography, oleoresins, carboxylic acids, dehydroabietic acid, neoabietic acid, palustric acid, pimaranes, abietanes, resin acids, levopimaric acid

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https://www.fs.usda.gov/treesearch/pubs/26853