Skip to Main Content
U.S. Forest Service
Caring for the land and serving people

United States Department of Agriculture

Home > Search > Publication Information

  1. Share via EmailShare on FacebookShare on LinkedInShare on Twitter
    Dislike this pubLike this pub
    Author(s): Kristina F. Connor; F.T. Bonner
    Date: 1998
    Source: Seed Technology. 20(1): 32-42
    Publication Series: Scientific Journal (JRNL)
    PDF: View PDF  (174.0 KB)

    Description

    Investigations of recalcitrant, or desiccation-sensitive, seeds have as yet failed to identify the causes of this phenomenon. Experiments with Guarea guidonia (L.) Sleumer (American muskwood) were initiated to determine the effects of desiccation on the physiology and biochemistry of the seeds of this tropical tree species. Seeds were air-dried at room temperature for 7 days. At intervals, germination was tested, moisture content determined, and lipids extracted. The bulk lipids, nonpolar lipids, monoglycerides, and phospholipids were analyzed by gas chromatography (GC), thermal characteristics of whole tissue samples were examined using differential scanning calorimetry (DSC), and moisture content was determined using the Karl Fisher analysis. DSC thermograms showed that as moisture content and germinability of seeds declined, so did enthalpy values and onset temperatures of cotyledon tissue and embryonic axes. GC analyses determined that unsaturated fatty acids accounted for approximately 70% of the bulk lipids; however, ratios of unsaturated/saturated fatty acids and amounts of individual fatty acids fluctuated between test periods. Palmitic acid was the most common saturated fatty acid, and linoleic acid was the most prevalent unsaturated fatty acid. Generally, Karl Fisher analyses of seed moisture content offered the best possibility of monitoring seed deterioration during drying.

    Publication Notes

    • You may send email to pubrequest@fs.fed.us to request a hard copy of this publication.
    • (Please specify exactly which publication you are requesting and your mailing address.)
    • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

    Citation

    Connor, Kristina F.; Bonner, F.T. 1998. Physiology and biochemistry of recalcitrant Guarea guidonia (L.) Sleumer seeds. Seed Technology. 20(1): 32-42.

    Keywords

    recalcitrant, seeds, storage

    Related Search


    XML: View XML
Show More
Show Fewer
Jump to Top of Page