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    Author(s): D.H. Slone; B.T. Sullivan
    Date: 2007
    Source: J. Chem. Ecol., Vol. 33: 1748-1762
    Publication Series: Miscellaneous Publication
    PDF: View PDF  (981 KB)

    Description

    Coupled gas chromatography/electroantennographic detection (GC-EAD) is a widely used method for identifying insect olfactory stimulants present in mixtures of volatiles, and it can greatly accelerate the identification of insect semiochemicals. In GC-EAD, voltage changes across an insect's antenna are measured while the antenna is exposed to compounds eluting fi-om a gas chromatograph. The antenna thus serves as a selective GC detector whose output can be compared to that of a "general" GC detector, commonly a flame ionization detector. Appropriate interpretation of GC-EAD results requires that olfaction-related voltage changes in the antenna be distinguishable from background noise that arises inevitably from antenna1 preparations and the GC-EAD-associated hardware. In this paper, we describe and compare mathematical algorithms for discriminating olfaction generated signals in an EAD trace from background noise. The algorithms amplify signals by recognizing their characteristic shape and wavelength while suppressing unstructured noise. We have found these algorithms to be both powerful and highly discriminatory even when applied to noisy traces where the signals would be difficult to discriminate by eye. This new nlethodology removes operator bias as a factor in signal identification, can improve realized sensitivity of the EAD system, and reduces the number of runs required to confirm the identity of an olfactory stimulant.

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    Citation

    Slone, D.H.; Sullivan, B.T. 2007. An automated approach to detecting signals in electroantennogram data. J. Chem. Ecol., Vol. 33: 1748-1762

    Keywords

    olfaction, antennogram, statistical model, semiochemical, signal-to-noise ratio

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