Skip to Main Content
Composition of the bacterial community in the gut of the pine engraver, Ips pini (Say) (Coloptera) colonizing red pineAuthor(s): Italo Jr. Delalibera; Archana Vasanthakumar; Benjamin J. Burwitz; Patrick D. Schloss; Kier D. Klepzig; Jo Handelsman; Kenneth F. Raffa
Source: Symbiosos, Vol. 43: 97-104
Publication Series: Miscellaneous Publication
PDF: View PDF (365 KB)
DescriptionThe gut bacterial community of a bark beetle, the pine engraver Ips pini (Say), was characterized using culture-dependent and culture-independent methods. Bacteria from individual guts of larvae, pupae and adults were cultured and DNA was extracted from samples of pooled larval guts. Analysis of 16S rRNA gene sequences amplified directly from the gut community suggests that the gut bacterial communities associated with I. pini are relatively simple, compared to many other systems. Six bacterial genera from four classes were detected by culturing gut bacteria from larvae, pupae and adults. Two genera, Pantoea and Stenotrophomonas (γ-Proteobacteria) were found in all life stages of I. pini, consistently in larvae and adults, and less commonly in pupae. Sequences that affiliate with the Enterobactereaceae of the γ-Proteobacteria were found in 95% of the clones sampled. The Enterobactereaceae genera, Pantoea and Erwinia, accounted for 88% of all clone sequences. These results are consistent with previous work indicating that another bark beetle, the southern pine beetle, Dendroctonus frontalis Zimmerman, also has a relatively simple gut flora, compared with wood colonizing insects such as wood borers and termites. The composition and abundance of bacteria associated with different life stages of I. pini are possibly associated with specific functions of the gut bacterial communities of larvae, pupae, and adults.
- You may send email to email@example.com to request a hard copy of this publication.
- (Please specify exactly which publication you are requesting and your mailing address.)
CitationDelalibera, Italo Jr.; Vasanthakumar, Archana; Burwitz, Benjamin J.; Schloss, Patrick D.; Klepzig, Kier D.; Handelsman, Jo; Raffa, Kenneth F. 2007. Composition of the bacterial community in the gut of the pine engraver, Ips pini (Say) (Coloptera) colonizing red pine. Symbiosos, Vol. 43: 97-104
Keywordsbark beetle, gut microbiota, 16s ribosomal RNA, rarefaction analysis
- The Trophic Significance of Bacteria in a Detritus-Based Stream Food Web
- Gut microbiota of an invasive subcortical beetle, Agrilus planipennis Fairmaire, across various life stages
- Endophytic bacteria in cacti seeds can improve the development of cactus seedlings.
XML: View XML