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Microsatellite DNA markers for delineating population structure and kinship among the endangered Kirtland’s warbler (Dendroica kirtlandii)Author(s): TIM L. KING; MICHAEL S. EACKLES; ANNE P. HENDERSON; CAROL I. BOCETTI; DAVE CURRIE; JR WUNDERLE
Source: Molecular Ecology Notes 5, 569–571
Publication Series: Miscellaneous Publication
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DescriptionWe document the isolation and characterization of 23 microsatellite DNA markers for the endangered Kirtland’s warbler (Dendroica kirtlandii), a Nearctic/Neotropical migrant passerine. This suite of markers revealed moderate to high levels of allelic diversity (averaging 7.7 alleles per locus) and heterozygosity (averaging 72%). Genotypic frequencies at 22 of 23 (95%) markers conformed to Hardy–Weinberg equilibrium expectations, and no linkage disequilibrium was observed in blood samples taken from 14 warblers found on the wintering grounds in the Bahamas archipelago. Multilocus genotypes resulting from this suite of markers should reduce the amount of resources required for initiating new genetic studies assessing breeding structure, parentage, demographics, and individual-level ecological interactions for D. kirtlandii.
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CitationKING, TIM L.; EACKLES, MICHAEL S.; HENDERSON, ANNE P.; BOCETTI, CAROL I.; CURRIE, DAVE; WUNDERLE, JR, JOSEPH M.k. 2005. Microsatellite DNA markers for delineating population structure and kinship among the endangered Kirtland’s warbler (Dendroica kirtlandii). Molecular Ecology Notes 5, 569–571
KeywordsKirtland’s warbler, microsatellite DNA, population structure, probability of identity
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