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    Author(s): TIM L. KING; MICHAEL S. EACKLES; ANNE P. HENDERSON; CAROL I. BOCETTI; DAVE CURRIE; JR WUNDERLE
    Date: 2005
    Source: Molecular Ecology Notes 5, 569–571
    Publication Series: Miscellaneous Publication
    PDF: View PDF  (75 B)

    Description

    We document the isolation and characterization of 23 microsatellite DNA markers for the endangered Kirtland’s warbler (Dendroica kirtlandii), a Nearctic/Neotropical migrant passerine. This suite of markers revealed moderate to high levels of allelic diversity (averaging 7.7 alleles per locus) and heterozygosity (averaging 72%). Genotypic frequencies at 22 of 23 (95%) markers conformed to Hardy–Weinberg equilibrium expectations, and no linkage disequilibrium was observed in blood samples taken from 14 warblers found on the wintering grounds in the Bahamas archipelago. Multilocus genotypes resulting from this suite of markers should reduce the amount of resources required for initiating new genetic studies assessing breeding structure, parentage, demographics, and individual-level ecological interactions for D. kirtlandii.

    Publication Notes

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    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

    Citation

    KING, TIM L.; EACKLES, MICHAEL S.; HENDERSON, ANNE P.; BOCETTI, CAROL I.; CURRIE, DAVE; WUNDERLE, JR, JOSEPH M.k. 2005. Microsatellite DNA markers for delineating population structure and kinship among the endangered Kirtland’s warbler (Dendroica kirtlandii). Molecular Ecology Notes 5, 569–571

    Keywords

    Kirtland’s warbler, microsatellite DNA, population structure, probability of identity

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