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    Author(s): SHARON A. CANTRELL
    Date: 2004
    Source: Caribbean Journal of Science, Vol. 40, No. 1, 8-16,
    Publication Series: Miscellaneous Publication
    PDF: Download Publication  (112 B)


    Most of the fungal diversity studies that have used a systematic collecting scheme have not included the discomycetes, so optimal sampling methods are not available for this group. In this study, I tested two sampling methods at each sites in the Caribbean National Forest, Puerto Rico and Ebano Verde Reserve, Dominican Republic. For a plot-based sampling method, 10 × 10 m plots were established and divided into one hundred 1 × 1 m subplots. For each sample, 12 subplots were selected at random with replacement. For a transect-based sampling method, 60 m long transects were established with twelve 1 × 1 m subplots randomly placed on either side of the transect line at 5 m intervals at the beginning of the study. The study was conducted from October 2001 to September 2002. For Puerto Rico, 46 and 51 morpho-species were identified in the transects and plots, respectively. There was a 32% overlap (68% complementarity) between sites. The Sorensen Similarity Coefficient between sites was 0.50 for both methods, and 0.55-0.63 between methods within sites. For the Dominican Republic, 25 and 26 morpho-species were identified in the transects and plots, respectively. There was a 24-31% overlap (69-76% complementarity) between sites. The Sørensen Similarity Coefficient between sites was 0.40-0.47 for transects and plots, respectively, and 0.40-0.70 between methods within sites. The species accumulation curve indicates that the minimum number of subplots needed is 10 per transect and 60-70 per plot to obtain between 70-80% of the species. In terms of sampling effort, I concluded that at least 12 samples distributed throughout a year but with shorter intervals during the rainy season are needed. There was no difference between using transects or plots based on the number of species and similarity indexes. Based on a Chi-Square analysis using the frequencies of species, however, transects were better that plots because the distribution of species is more homogeneous.

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    CANTRELL, SHARON A. 2004. A Comparison of Two Sampling Strategies to Assess Discomycete Diversity in Wet Tropical Forests. Caribbean Journal of Science, Vol. 40, No. 1, 8-16,


    discomycetes, Ascomycota, Fungi, sampling methods, diversity, tropical, Caribbean

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