Skip to Main Content
U.S. Forest Service
Caring for the land and serving people

United States Department of Agriculture

Home > Search > Publication Information

  1. Share via EmailShare on FacebookShare on LinkedInShare on Twitter
    Dislike this pubLike this pub
    Author(s): W. Sutton; E.M. Hansen; P. Reeser; A. Kanaskie
    Date: 2008
    Source: In: Frankel, Susan J.; Kliejunas, John T.; Palmieri, Katharine M., tech. coords. 2008. Proceedings of the sudden oak death third science symposium. Gen. Tech. Rep. PSW-GTR-214. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station. pp. 467-470
    Publication Series: General Technical Report (GTR)
    Station: Pacific Southwest Research Station
    PDF: View PDF  (183 KB)

    Description

    Stream monitoring using leaf baits for early detection of P. ramorum is an important part of the Oregon sudden oak death program. About 50 streams in and near the Oregon quarantine area in the southwest corner of the state are currently monitored. Rhododendron and tanoak leaf baits in mesh bags are exchanged every two weeks throughout the year. Leaves are sent to Corvallis for isolation and PCR diagnosis using the Winton and Hansen ITS primers. P. ramorum is regularly recovered from streams draining infested sites 5 years after eradication treatment. Because there has been a very good correspondence between culture results and PCR results rom these known infested streams, we have changed to PCR only diagnosis on streams outside the known diseased area. If a PCR positive result is obtained, these baits and subsequent baits from that stream are cultured as well as assayed by PCR. In three watersheds Phytophthora ramorum was first detected using stream baiting. Follow up intensive ground surveys located infected tanoaks or other host plants. Phytophthora ramorum has been cultured from one additional stream but a source of inoculum has not yet been found.

    Publication Notes

    • You may send email to rmrspubrequest@fs.fed.us to request a hard copy of this publication.
    • (Please specify exactly which publication you are requesting and your mailing address.)
    • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

    Citation

    Sutton, W.; Hansen, E.M.; Reeser, P.; Kanaskie, A. 2008. Stream monitoring for detection of Phytophthora ramorum in Oregon. In: Frankel, Susan J.; Kliejunas, John T.; Palmieri, Katharine M., tech. coords. 2008. Proceedings of the sudden oak death third science symposium. Gen. Tech. Rep. PSW-GTR-214. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station. pp. 467-470

    Keywords

    Phytophthora ramorum, sudden oak death, PCR, stream monitoring

    Related Search


    XML: View XML
Show More
Show Fewer
Jump to Top of Page