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    Author(s): U.P. Agarwal; R.H. Atalla
    Date: 1986
    Source: Planta. Vol. 169 (1986): Pages 325-332.
    Publication Series: Miscellaneous Publication
    PDF: View PDF  (130 KB)

    Description

    Native-state organization and distribution of cell-wall components in the secondary wall of woody tissue from P. mariana (Black Spruce) have been investigated using polarized Raman microspectroscopy. Evidence for orientation is detected through Raman intensity variations resulting from rotations of the exciting electric vector with respect to cell-wall geometry. Spectral features associated with cellulose and lignin were studied. The changes in cellulose hands indicate that the pyranose rings of the anhydroglucose repeat units are in planes perpendicular to the cross section, while methine C-H bonds are in planes parallel to the cross section. Changes in bands associated with lignin indicate that the aromatic rings of the phenyl-propane units are most often in the plane of the cell-wall surface. However, regions where lignin orientation departs from this pattern also occur. These results represent direct evidence of molecular organization with respect to cellular morphological features in woody tissue, and indicate that cell-wall components are more highly organized than had been recognized. Studies carried out in order to establish the usefulness and sensitivity of the Raman technique to differences of composition within the cell walls provide evidence of variations in the distribution of cellulose and lignin. Such compositional differences were more prominent between the walls of different cells than within a particular cell wall.

    Publication Notes

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    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

    Citation

    Agarwal, U.P.; Atalla, R.H. 1986. In-situ Raman microprobe studies of plant cell walls: macromolecular organization and compositional variability in the secondary wall of Picea mariana (Mill.) B.S.P. Planta. Vol. 169 (1986): Pages 325-332.

    Keywords

    Cell wall structure, cellulose, Coniferae, lignin, Picea, Raman microprobe, orientation, spectrum analysis, black spruce, plant cell walls, Raman spectroscopy, ultrastructure, wood chemistry, cellulose chemistry

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