Skip to Main Content
An improved extraction method to increase DNA yield from molted feathersAuthor(s): Shelley Bayard De Volo; Richard T. Reynolds; Marlis R. Douglas; Michael F. Antolin
Source: The Condor. 110(4): 762-767.
Publication Series: Miscellaneous Publication
PDF: View PDF (255 B)
DescriptionTo assess the value of molted feathers as a noninvasive source of DNA for genetic studies of Northern Goshawks (Accipiter gentilis), we isolated and quantified DNA from molted feathers and compared yields across five feather types. We also compared PCR success across the same five feather types using five microsatellite genetic markers of varying size. In addition, we compared DNA yields from a commonly used extraction method versus one we modified to increase DNA yield. Results indicated molted feathers provided on average 24 ng ?l-1 of DNA, which is a relatively high DNA yield compared to other noninvasive tissue sources. Tail feathers yielded significantly more DNA than primary, secondary, and smaller feathers, yet all feather sizes produced equally high rates of PCR success. Although our modified extraction method increased the time required for processing feathers, it resulted in significantly higher yields of DNA as compared to the unmodified protocol.
- You may send email to firstname.lastname@example.org to request a hard copy of this publication.
- (Please specify exactly which publication you are requesting and your mailing address.)
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
CitationBayard De Volo, Shelley; Reynolds, Richard T.; Douglas, Marlis R.; Antolin, Michael F. 2008. An improved extraction method to increase DNA yield from molted feathers. The Condor. 110(4): 762-767.
KeywordsAccipiter gentilis, DNA extraction, DNA yield, molted feathers, noninvasive genetic sampling, Northern Goshawk
- Direct amplification of DNA from fresh and preserved ectomycorrhizal root tips
- Multiplex sequencing of plant chloroplast genomes using Solexa sequencing-by-synthesis technology
- Population genetics and genotyping for mark-recapture studies of Northern Goshawks (Accipiter gentilis) on the Kaibab Plateau, Arizona
XML: View XML